Search for dissertations about: "real-time quantitative PCR"

Showing result 1 - 5 of 102 swedish dissertations containing the words real-time quantitative PCR.

  2. 1. Kinetics Quality Assessment for real-time PCR

    Author : Tzachi Bar; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

    Abstract : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. READ MORE

  4. 2. Pre-PCR Processing Strategies for Quantitative Detection of Food-Borne Pathogens using Real-Time PCR

    Author : Petra Wolffs; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; DNA polymerase; sample treatment; pre-PCR processing; real-time PCR; Food-borne pathogens;

    Abstract : Since the development of real-time PCR, the technology has been widely applied in the field of diagnostics. In comparison with conventional PCR it has opened up the possibility of accurate quantification of microorganisms in clinical, environmental and food samples. READ MORE

  5. 3. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Author : Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
    Keywords : Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Abstract : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. READ MORE

  6. 4. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    Author : Rickard Knutsson; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE

  7. 5. Acute gastroenteritis in Rwandan children under five years of age investigated by real-time PCR

    Author : Jean-Claude Kabayiza; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Gastroenteritis; Real-time PCR;

    Abstract : Acute gastroenteritis is a major cause of illness and death among children in developing countries. Knowledge about the aetiology is important to make the right priorities regarding preventive measures, and for the recommendation to use or not use antibiotics. READ MORE