Search for dissertations about: "recombinant DNA technology"

Showing result 1 - 5 of 34 swedish dissertations containing the words recombinant DNA technology.

2. 1. Recombinant Enzymes in Pyrosequencing Technology

   Author : Nader Nourizad; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

4. 2. Gene technology at stake : Swedish governmental commissions on the border of science and politics

   Author : Jenny Eklöf; Kjell Jonsson; Christer Nordlund; Susanne Lundin; Umeå universitet; []
   Keywords : HUMANIORA; HUMANITIES; Gene technology; biotechnology; recombinant DNA technology; bioethics; ethics; risk; GMO; embryo; transgenic organisms; boundary organization; boundary object; governmental commission; regulatory policy; Sweden; public; expertise; democracy; History of science and ideas; Idé- o lärdomshistoria;

   Abstract : This thesis examines the Swedish political response to the challenges posed by gene technology, seen through the prism of governmental commissions. It discerns and analyses continuities and changes in the Swedish political conception of gene technology, over the course of two decades, 1980–2000. READ MORE

5. 3. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

   Author : Zihe Liu; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

   Abstract : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. READ MORE

6. 4. Novel Moderate Halophiles: Application of Halomonas boliviensis in Biopolyester Production

   Author : Jorge Quillaguaman; Bioteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; biopolymers; Polymer technology; poly b-hydroxybutyrate accumulation; moderate halophile; Halomonas boliviensis; Chromohalobacter sarecencis; Polymerteknik; Bioteknik;

   Abstract : The south-west Andean region of Bolivia has vast landscapes comprising salt deserts and dry lands interspersed with a number of saline lakes. Much of this region lies above 4000 m above sea level that allows the survival of limited and unique flora and fauna. READ MORE

7. 5. Xylan degradation by the thermophilic bacterium Rhodothermus marinus. Characterization and function of a thermostable xylanase

   Author : Eva Nordberg Karlsson; Bioteknik; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fed-batch cultivation; kraft pulp; bleaching; glycosyl hydrolase family 10; recombinant xylanase; polysaccharide binding domain; Biotechnology; Bioteknik;

   Abstract : A gene encoding a multidomain xylanase from the thermophilic bacterium Rhodothermus marinus has been cloned, sequenced and produced in Escherichia coli . The gene product was found to be a 109 kDa protein consisting of five domains. READ MORE