Search for dissertations about: "recombinant protein from e.coli"

Showing result 1 - 5 of 63 swedish dissertations containing the words recombinant protein from e.coli.

  2. 1. Recombinant amelogenin - Strategies for purification and protein processing

    Author : Johan Svensson Bonde; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

    Abstract : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. READ MORE

  4. 2. Combinatorial Protein Engineering Of Affibody Molecules Using E. Coli Display And Rational Design Of Affibody-Based Tracers For Medical Imaging

    Author : Ken G. Andersson; John Löfblom; Stefan Ståhl; Harald Kolmar; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; directed evolution; microbial display; E. coli; Affibody molecule; autotransporter; medical imaging; HER receptor family; Sortase A; Bioteknologi; Biotechnology;

    Abstract : Directed evolution is today an established strategy for generation of new affinity proteins. This thesis describes the development of a cell-display method using Escherichia coli for directed evolution of Affibody molecules. READ MORE

  5. 3. HYDROPHOBIC PEPTIDE TAGS - As tools in bioseparation and investigation of recombinant proteins

    Author : Sara Fexby; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; E. coli; Biochemical technology; Biokemisk teknik; fusion protein; tyrosine; hydrophobicity; Hydrophobic interaction chromatography; aqueous two-phase system;

    Abstract : This thesis describes the possibility of using hydrophobic peptide tags as tools for bioseparation and for the investigation of recombinant proteins. The hydrophobicity of a protein can easily be changed by minor modifications, such as fusions of tyrosine-containing peptides. READ MORE

  6. 4. Redox balancing in recombinant strains of Saccharomyces cerevisiae

    Author : Mikael Anderlund; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; xylose isomerase; xylitol dehydrogenase; xylose reductase; glycerol; xylitol; xylose; E. coli; A. vinelandii; T. thermophilus; S. cerevisiae; P. stipitis; transhydrogenase.; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : In metabolically engineered Saccharomyces cerevisiae expressing Pichia stipitis XYL1 and XYL2 genes, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, xylitol is excreted as the major product during anaerobic xylose fermentation and only low yields of ethanol are produced. This has been interpreted as a result of the dual cofactor dependence of XR and the exclusive use of NAD+ by XDH. READ MORE

  7. 5. Expression and structure-function characterisation of herpesviral proteins

    Author : Sue-Li Dahlroth; Pär Nordlund; Darren Hart; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein production; Herpesvirus; SOX; E. coli; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : In order to determine and study a protein structure, large amounts of it is needed. The easiest way to obtain a protein is to recombinantly overexpress it in the well-studied bacterium Escherichia coli. However, this expression host has one major disadvantage, overexpressed proteins might not be folded or be insoluble. READ MORE