Search for dissertations about: "recombinant protein production in E.coli thesis"

Showing result 1 - 5 of 45 swedish dissertations containing the words recombinant protein production in E.coli thesis.

2. 1. Protein production in the E. coli cell envelope

   Author : Thomas Baumgarten; Jan-Willem de Gier; Dirk-Jan Scheffers; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; Biochemistry; biokemi;

   Abstract : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. READ MORE

4. 2. Membrane and secretory protein production in Escherichia coli

   Author : Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

   Abstract : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. READ MORE

5. 3. Enhancing membrane and secretory protein production yields in Escherichia coli

   Author : Zhe Zhang; Jan-Willem de Gier; Claes von Wachenfeldt; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; recombinant protein production; Escherichia coli; membrane protein; secretory protein; strain engineering; Biochemistry; biokemi;

   Abstract : Proteins fulfill essential functions in living cells. To produce sufficient amounts of a protein is essential to study the structure and function of a protein, or to use it for medical purposes. Escherichia coli is a Gram-negative bacterium that is widely used for recombinant protein production. READ MORE

6. 4. Recombinant amelogenin - Strategies for purification and protein processing

   Author : Johan Svensson Bonde; Tillämpad biokemi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

   Abstract : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. READ MORE

7. 5. Expression and structure-function characterisation of herpesviral proteins

   Author : Sue-Li Dahlroth; Pär Nordlund; Darren Hart; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Protein production; Herpesvirus; SOX; E. coli; Biochemistry; Biokemi; biokemi; Biochemistry;

   Abstract : In order to determine and study a protein structure, large amounts of it is needed. The easiest way to obtain a protein is to recombinantly overexpress it in the well-studied bacterium Escherichia coli. However, this expression host has one major disadvantage, overexpressed proteins might not be folded or be insoluble. READ MORE