Search for dissertations about: "refolding"

Showing result 1 - 5 of 27 swedish dissertations containing the word refolding.

  2. 1. Interaction engineered three-helix bundle domains for protein recovery and detection

    Author : Tove Alm; Sophia Hober; Rainer Hahn; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ABD; Zbasic; albumin; protein engineering; phage display; staphylococcal display; inclusion bodies; refolding; proteomics; orthogonal affinity purification; Bioengineering; Bioteknik; Immunology engineering; Immunteknik;

    Abstract : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. READ MORE

  4. 2. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE

  5. 3. Conformational Studies of Protein Structure and Dynamics

    Author : Dick Andersson; Franz X. Schmid; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The protein folding problem is one of the most important issues to be solved in the field of molecular biology. The subject of this thesis mainly deals with various aspects of the folding process. READ MORE

  6. 4. The Interaction Between the Chaperonin GroEL and Human Carbonic Anhydrase II : Protein Folding and Stability Studies

    Author : Malin Persson; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; carbonic anhydrase; protein folding; protein stability; chaperone; GroEL; folding mechanism; site-directed spinlabeling; electron paramagnetic resonance EPR ;

    Abstract : The presence of GroEL/ES during the refolding of human carbonic anhydrase II was found to increase the yield of active enzyme from approx. 70 to approx. 100%. This chaperone action on the enzyme could be obtained by adding GroEL alone, and the time course in that case was only moderately slower than the spontaneous process. READ MORE

  7. 5. Rate Limiting Factors For Protein Folding

    Author : Maria Berggård Silow; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; kinetic competition; transient aggregation; diffusion control; reconfiguration time; collapse; osmolytes; broad barriers; Hammond behaviour; two-state; U1A; CI2; Proteins; enzymology; Proteiner; enzymologi;

    Abstract : Abstract. This thesis describes factors that are rate limiting for the folding of two small proteins, U1A and CI2 which fold without accumulating intermediates. The [GdnHCl] dependencies of the unfolding- and refolding kinetics of U1A display downward curvatures. READ MORE