Search for dissertations about: "single-molecule detection"

Showing result 1 - 5 of 43 swedish dissertations containing the words single-molecule detection.

  2. 1. Single Molecule Detection : Microfluidic Automation and Digital Quantification

    Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

  4. 2. Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques

    Author : Ida Grundberg; Mats Nilsson; Ulf Landegren; Olli-Pekka Kallioniemi; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; padlock probes; in situ; rolling circle amplification; mRNA; genotyping; mutation detection; cancer; tissue sections; diagnostics; single-molecule; single-cell; microscopy; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

    Abstract : The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. READ MORE

  5. 3. Single-molecule Detection in situ

    Author : Chatarina Larsson; Mats Nilsson; Ulf Landegren; Sanjay Tyagi; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Molekylär medicin; Molecular Medicine; molekylär medicin genetik och patologi ;

    Abstract : The human body contains a variety of different cell types that share a common genome, but differ in how they use the information encoded therein. Variation in molecular content exists even at the level of individual cells, and to provide deeper insight into complex cellular processes methods that permit analysis of each cell on its own are needed. READ MORE

  6. 4. Probing Biomolecular Recognition at the Single Molecule Level

    Author : Anders Gunnarsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cell membrane; TIRFM; liposome; time-of-flight secondary ion mass spectrometry; membrane receptor; single molecule; TOF-SIMS; molecular recognition; DNA; barcode; total internal reflection; multiplexing;

    Abstract : Specific recognition between biomolecular partners contributes to a multitude of biochemical signaling within and between cells. The aim of this work has been to investigate the possibility to probe biomolecular recognition reactions at the smallest possible scale, the single molecule level. READ MORE

  7. 5. Single-Molecule Detection and Optical Scanning in Miniaturized Formats

    Author : Jonas Melin; Fredrik Nikolajeff; Mats Nilsson; Hugh Fan; Uppsala universitet; []
    Keywords : Biotechnology; microfluidics; single-molecule detection; MOEMS; μTAS; lab-on-a-chip; RCA; thermoplastics; injection molding; hot embossing; Bioteknik;

    Abstract : In later years polymer replication techniques have become a frequently employed fabrication method for microfluidic and micro-optical devices. This thesis describes applications and further developments of microstructures replicated in polymer materials. READ MORE