Search for dissertations about: "single-molecule tracking"

Showing result 1 - 5 of 8 swedish dissertations containing the words single-molecule tracking.

  2. 1. Tracking single molecules in uncharted territory : A single-molecule method to study kinetics in live bacteria

    Author : Javier Aguirre Rivera; Magnus Johansson; Dmitri N. Ermolenko; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; single-molecule; protein synthesis; fluorescence microscopy; antibiotics; aminoglycosides; macrolides; apramycin; gentamicin; paromomycin; S13; translation; bacteria; Molekylär biovetenskap; Molecular Life Sciences;

    Abstract : The synthesis of proteins, also known as translation, is a fundamental process in every living organism. The steps in the translation of genetic information to functional proteins have been meticulously studied, mostly using in vitro techniques, yielding a detailed model of their mechanisms. READ MORE

  4. 2. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Author : Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Abstract : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. READ MORE

  5. 3. Multi-dimensional imaging : A tool to unravel material properties at the nanoscale

    Author : Boris Louis; NanoLund: Centre for Nanoscience; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Optical microscopy; Fluorescence Microscopy; Multi-dimensional imaging; Material sciences; Optical Trapping; Single-molecule imaging and tracking; Single-molecule spectroscopy;

    Abstract : Optical microscopy has revolutionized the way we understand our world and has become a crucial tool in a range of disciplines such as biology, physics, chemistry, and material sciences. The development of new microscopy techniques has been capital to its success and wide applicability. READ MORE

  6. 4. Orientational dynamics of single molecules applied to protein-DNA target search

    Author : Emil Marklund; Johan Elf; Erwin Peterman; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; single molecule imaging; fluorescence correlation spectroscopy; gene regulation; transcription factor; lac operon;

    Abstract : Cells in all life forms regulate their genes with the help of transcription factors (TFs). These proteins turn genes on or off by binding regulatory motifs present in the DNA. Before this binding can occur, a TF needs to search and find the specific binding site in the genome of the cell. READ MORE

  7. 5. Multidimensional Fluorescence Polarization Imaging of Single Light Harvesting Complexes

    Author : Sumera Tubasum; Kemisk fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Light Harvesting Complexes; Single Molecule Spectroscopy; Polarization Spectroscopy; Exciton Dynamics; Spectral Disorder;

    Abstract : This thesis presents my research journey in the Department of Chemical Physics,Lund University. Multidimensional fluorescence polarization imaging is used to study single LH2s. The method uses linearly polarized excitation light and the emission is detected through a polarizer. READ MORE