Search for dissertations about: "solubility protein E.coli thesis"
Showing result 1 - 5 of 8 swedish dissertations containing the words solubility protein E.coli thesis.
-
1. Synthesis and functionalization of ring-fused 2-pyridones : Targeting pili formation in E. coli
Abstract : Bicyclic dihydrothiazolo fused 2-pyridones have been studied as a new class of antibacterial agents, termed pilicides, that target the formation of adhesive bacterial surface organelles (pili) in pathogenic bacteria. Synthetic methods to further functionalize the bicyclic 2-pyridone scaffold have been developed in order to increase water-solubility and thereby facilitate biological evalua-tions. READ MORE
-
2. Protein production, characterization and structure determination in structural genomics
Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE
-
3. Probing the prospects for structural genomics : expression and characterization of human disease-related proteins in E. coli
Abstract : Today a lot of research is directed towards the development of methods to make protein structure determination faster and more generalised. Automated, general methods for protein expression and purification, robotics for crystallisation and automatic methods for data collection will take structural biology into a new era, that of structural genomics. READ MORE
-
4. Protein production and purification in structural genomics
Abstract : The number of gene products available for structural and functional study is increasing at an unprecedented rate as a result of the successful whole genome sequencing projects. Systematic structure determination of proteins on a genomic scale, called structural genomics, can significantly contribute to the field of protein science and to functional annotation of newly identified genes. READ MORE
-
5. Methodology for high-throughput production of soluble recombinant proteins in Escherichia coli
Abstract : The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. READ MORE