Search for dissertations about: "[3H]MK-801 binding"

Found 3 swedish dissertations containing the words [3H]MK-801 binding.

  2. 1. Regulation of NMDA receptor properties by amino acids and cations : a biochemical and electrophysiological study

    Author : Yun Liu; Karolinska Institutet; Karolinska Institutet; []
    Keywords : NMDA receptor; Glutamate; Glycine; [3H]MK-801 binding; Magnesium; Calcium; Tris; Potassium; Sodium; Proton; Xenopus oocytes; Spinal cord; Cerebral cortex; Receptor binding; Electrophysiology;

    Abstract : NMDA receptors are glutamate-regulated ion channels that are permeable to Ca2+, Na+ and K+ and are sensitive to voltage-dependent Mg2+-block. To investigate the modulatory effects of amino acids and cations on NMDA receptors and the possible regional differences in the pharmacology of NMDA receptors, I used mainly two techniques in this thesis: electrophysiological analysis of expressed NMDA receptors in Xenopusoocytes and biochemical receptor binding using [3H]MK-801 in membrane preparations from the rat cerebral cortex and spinal cord. READ MORE

  4. 2. Effects of ethanol on NMDA receptor-mediated functions in primary cultures of cerebellar granule cells

    Author : Aleta Cebere; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Glutamate; NR2A and NR2B subunits; NMDA ethanol; homoquinolinic acid; glycine; spermidine; NMDA receptor antagonists; Ca 21; neurotoxicity; neuroprotection; AP-1; RT-PCR; Western blot; cerebellar granule cells;

    Abstract : Primary cultures of cerebellar granule cells were used to investigate the effects of acute and chronic exposure of ethanol on NMDA receptor functions and to examine the possible sites of interaction between ethanol and the NMDA receptor. Activation of NMDA receptors induced elevation of free cytoplasmic Ca2+, Ca2+ influx, activation of protein kinase C and neurotoxicity. READ MORE

  5. 3. Human spinal cord transplantation : experimental and clinical application

    Author : Elisabet Åkesson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The main objectives of this Thesis was to further characterize the in situ human first trimester spinal cord tissue and to evaluate the survival, integration and possible neurological effects of solid human embryonic spinal cord grafts transplanted to cavities in the spinal cord of rat and man. Human-specific neurofilament and Thy-1 positive fibers were observed already at 5 weeks of gestation in the in situ human spinal cord, the earliest stage at which donor tissue was used for intraspinal transplantation. READ MORE