Search for dissertations about: "2D BN SDS-PAGE"

Found 5 swedish dissertations containing the words 2D BN SDS-PAGE.

2. 1. Membrane Protein Biogenesis in Escherichia coli : A proteomics approach

   Author : David Vikström; Jan-Willem de Gier; Eitan Bibi; Stockholms universitet; []
   Keywords : SRP; YidC; SecE; Membrane protein biogenesis; protein targeting; 2D BN SDS-PAGE; Biochemistry; biokemi;

   Abstract : In the bacterium Escherichia coli all proteins are synthesized in the cytoplasm. However, at least 25% of them then need to be exported to another cellular compartment where they exert their function. E. coli has several different targeting pathways to ensure the correct localization of its proteins. READ MORE

4. 2. Protein interactions in signaling networks in Arabidopsis

   Author : Simone Schopper; Molekylär cellbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : Protein networks drive numerous crucial processes in cells. One important component in their coordination is protein-protein interactions. Mitogen activated protein kinase (MAPK) signaling substrates and endoplasmic reticulum (ER) proteins in Arabidopsis were investigated with respect to their respective interactions. READ MORE

5. 3. A model for membrane organization and protein sorting in the cyanobacterium Synechocystis sp. PCC 6803

   Author : Tatiana Pisareva; Birgitta Norling; Colin Robinson; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; biokemi;

   Abstract : Cyanobacteria constitute a unique group of eubacteria, possessing outer and plasma membranes as well as internal thylakoid membranes, the site for both photosynthesis and respiration. The combination of sucrose density centrifugation and two-phase partitioning methods leads to purification of different membranes from the cyanobacterium Synechocystis sp. READ MORE

6. 4. Regulation of chloroplast development during the greening process

   Author : Yan Ji; Åsa Strand; Eevi Rintamäki; Umeå universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Arabidopsis thaliana; light response; anterograde signalling; plastid transcription; chloroplast development; photosynthesis; bZIP; PEP complex; de-etiolation; molekylärbiologi; Molecular Biology;

   Abstract : Upon light exposure, the expression of one-third of the nuclear-encoded genes is changing, including genes encoding many chloroplast-targeted proteins responsible for the initiation of plastid transcription and the establishment of photosynthesis. The control of the nuclear-encoded genes involved in the development of chloroplast is referred to as anterograde signalling. READ MORE

7. 5. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

   Author : Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

   Abstract : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. READ MORE