Search for dissertations about: "3D cell culture"

Showing result 16 - 20 of 77 swedish dissertations containing the words 3D cell culture.

  2. 16. Strength in Numbers – Droplet Microfluidics for Multicellular Ensemble Applications

    Author : Martin Trossbach; Aman Russom; Håkan Jönsson; Nicole Pamme; KTH; []
    Keywords : Droplet Microfluidics; High-Throughput Screening; Microtissues; Microreactors; Cell Factories; Cell Culture; Biomaterials; Spheroids; Biotechnology; Bioteknologi;

    Abstract : The work presented in this doctoral thesis explores multicellular, biological, and biotechnological applications for microfluidic droplets, making use of a number of unique features of these miniaturized, highly scalable reaction vessels.Droplet microfluidics specializes in pico- to nanoliter sized aqueous droplets in an immiscible oil phase, borrowing techniques from the field of microfluidics, namely fluid actuation, detection systems and electronic peripherals. READ MORE

  4. 17. Utilizing Biopolymers in 3D Tumor Modeling and Tumor Diagnosis

    Author : Negar Abbasi Aval; Torbjörn Pettersson; Aman Russom; Maria Tenje; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; 3D tumor modeling; Spheroids; Cellulose Nano Fibrils; Layer by Layer; Extracellular matrix; Circulating Tumor Capturing; Pancreatic ductal adenocarcinoma PDAC ; 3D-tumörmodellering; Sfärer; Cellulosa; Lager-på-lager; Extracellulärmatris; Infångning av cirkulerande tumörceller; Mänskliga Duktala Adenocarcinom-Hepatocyter; Fiber- och polymervetenskap; Fibre and Polymer Science;

    Abstract : Cancer represents a significant global public health challenge and ranks as the second mostcommon cause of death in the United States. The onset of cancer entails an initial phasewhere cells lose their polarity and disconnect from the normal basement membrane, allowingthem to form distinct three-dimensional (3D) configurations that interact with adjacent cellsand the surrounding microenvironment. READ MORE

  5. 18. Digital Image Analysis of Cells : Applications in 2D, 3D and Time

    Author : Amalka Pinidiyaarachchi; Carolina Wählby; Ewert Bengtsson; Arvid Lundervold; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Digital image analysis; microscopy; fluorescent staining; watershed segmentation; sub-cellular localization; point-like signals; wavelets; cell confluence; cytology; color spaces.; Image analysis; Bildanalys;

    Abstract : Light microscopes are essential research tools in biology and medicine. Cell and tissue staining methods have improved immensely over the years and microscopes are now equipped with digital image acquisition capabilities. The image data produced require development of specialized analysis methods. READ MORE

  6. 19. Regulation of hematopoiesis in the freshwater crayfish, Pacifastacus leniusculus : role of transglutaminase

    Author : Kingkamon Junkunlo; Irene Söderhäll; Kenneth Söderhäll; Chadanat Noonin; Mats W Johansson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Ast1; clotting protein; crayfish; hematopoiesis; PVF PVR; ROS; transglutaminase; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi; Biology with specialization in Comparative Physiology; Biologi med inriktning mot jämförande fysiologi;

    Abstract : The freshwater crayfish, Pacifastacus leniusculus, has been used as a model for studying hematopoiesis or blood cell production or hematopoiesis and immunity. The work of this thesis aims to investigate the impact of factors such as ROS signaling, Ast1, and the PVF/PVR signaling pathway in controlling stem cell behavior during hematopoiesis and specifically the role of the crosslinking enzyme transglutaminase (TGase) in regulation of hematopoiesis. READ MORE

  7. 20. Human iPSC-based models of theCNS: attaining cellular biofidelitythrough conventional and advancedculture systems

    Author : Dimitrios Voulgaris; Anna Herland; Susanna Narkhilakhi; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; neural stem cells; astrocytes; iPSC; hiAstrocytes; microphysiological systems; BBB; Bioteknologi; Biotechnology;

    Abstract : Brain development is a highly orchestrated process that entails changes in microenvironmental cues and growth factor gradients, which set the tempo for proper development of the rudimentary structures of the brain and the generation of neurons,astrocytes, and oligodendrocytes. Another intricate feature of the brain is the bloodbrain barrier (BBB). READ MORE