Search for dissertations about: "ACTIVE-SITE"

Showing result 1 - 5 of 378 swedish dissertations containing the word ACTIVE-SITE.

2. 1. Naphthoxylosides – Probing the β4GalT7 active site

   Author : Anna Siegbahn; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES;

   Abstract : Proteoglycans (PGs) is a class of highly anionic macromolecules that consist of one or more linear polysaccharide chains (glycosaminoglycans, GAGs) covalently attached to a core protein. The biological functions of PGs are mainly due to the interactions of GAG chains with various protein ligands and regulatory factors, such as cytokines and growth factors. READ MORE

4. 2. Characterization of Epoxide Hydrolases from Yeast and Potato

   Author : Lisa Tronstad-Elfström; Mikael Widersten; Michael Arand; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Epoxide hydrolase; Catalytic residues; Active site; trans-stilbene oxide; Rapid kinetics; Active site tyrosyls; Enzyme mechanism; Lewis acid; X-ray crystallography; Substrate specificity; Unidentified ORF; α β hydrolase fold; Saccharomyces cerevisiae; Biokemi; Biochemistry; Biokemi;

   Abstract : Epoxides are three-membered cyclic ethers formed in the metabolism of foreign substances and as endogenous metabolites. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to yield the corresponding diols. READ MORE

5. 3. Synthetic [FeFe] Hydrogenase Active Site Model Complexes

   Author : Lennart Schwartz; Sascha Ott; Marcetta Y. Darensbourg; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; hydrogenase mimic; proton reduction; bioinorganic chemistry; diiron hexacarbonyl complexes; artifical photosynthesis; hydrogen; Organic chemistry; Organisk kemi;

   Abstract : [FeFe]-Hydrogenases (H2ases) are metalloenzymes that can catalyze the reversible reduction of protons to molecular hydrogen as part of the metabolism of certain cyanobacteria and green algae. Due to the low availability of the enzyme, synthetic complexes that mimic the natural active site in structure, function and activity are highly sought after. READ MORE

6. 4. Poly(A)-Specific Ribonuclease (PARN)

   Author : Yan-Guo Ren; Eric Westhof; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Poly A -specific ribonuclease; PARN; deadenylation; processive; oligomeric; cap interacting; aminoglycoside; active site; FE2 -mediated cleavage; metal ion switch; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; Molekylär cellbiologi; Molecular Cellbiology;

   Abstract : Degradation of the mRNA 3'-end located poly(A) tail is an important step for mRNA decay in mammalian cells. Thus, to understand mRNA decay in detail, it is important to identify the catalytic activities involved in degrading poly(A). READ MORE

7. 5. Regulation of Ovarian Aromatase: Studies by Aromatase Assays in vitro and in vivo

   Author : Dmitrijus Kirilovas; Mats Brännström; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Obstetrics and gynaecology; aromatase; human; granulosa cells; 11C-vorozole; active site; allosteric configuration; in vitro; in vivo; PET; androgens; rat; estrous cycle.; Obstetrik och kvinnosjukdomar; Obstetrics and women s diseases; Obstetrik och kvinnosjukdomar; Obstetrics and Gynaecology; obstetrik och gynekologi;

   Abstract : An in vitro method was developed for measuring aromatase, based on binding of competitive aromatase inhibitor [11C]vorozole to the active site of the enzyme. [11C]Vorozole displayed high, specific binding in vitro to human placenta and human granulosa cells (GC), both fresh and frozen/thawed cells, provided correct procedures were used. READ MORE