Search for dissertations about: "Affinitetskromatografi"

Found 5 swedish dissertations containing the word Affinitetskromatografi.

  1. 1. Development of Flourescence-based Immunosensors for Continous Carbohydrate Monotoring Applications for Maltose and Glucose

    Author : Henrik Engström; Högskolan i Kalmar.; [2007]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Carbohydrate; Diabetes; Immunosensor; Glucose; Maltose; Weak affinity chromatograhpy; Kolhydrater; Glukos; Maltose; Affinitetskromatografi; Diabetes; MEDICINE Chemistry Biochemistry; MEDICIN Kemi Biokemi; Bioteknik; Biotechnology;

    Abstract : Weak affinity interaction of monoclonal antibodies and carbohydrate antigens can be detected and quantified by alterations in the antibodies' intrinsic tryptophan fluorescence. These weak/transient binding events have been monitored by total internal reflection fluorescence (TlRF) by facilitating the change in intrinsic tryptophan fluorescence. READ MORE

  2. 2. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r

    University dissertation from Viveka Dolby, Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-221 00 Lund

    Author : Viveka Dolby; Lund University.; Lunds universitet.; [2004]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; enzymology; Proteiner; enzymologi; Proteins; melanocortin 4 receptor; GPCR; purification; Expression; membrane proteins;

    Abstract : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. READ MORE

  3. 3. Modeling of Hindered Diffusion in Gels

    University dissertation from Department of Chemical Engineering, Lund University

    Author : Jan Gutenwik; Lund University.; Lunds universitet.; [2003]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; effective diffusion coefficient; pore size distribution; obstruction; immobilization; modeling; adsorption kinetics; hindered diffusion; simulation program; proteins; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

    Abstract : Hindered diffusion occurs in the most different processes, as has been described in this thesis. It is very important to have an understanding of the influence of hindered diffusion on the mass transfer rate, especially when competing rate processes are acting simultaneously. READ MORE

  4. 4. Development of low affinity techniques for applications in clinical chemistry

    University dissertation from Department of Chemistry, Lund University

    Author : Lisa Leickt; Lund University.; Lunds universitet.; [2001]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; chromatography performance; acute myocardial infarction; antibodies; creatine kinase MB isoforms; lectins; low affinity interactions; weak affinity chromatography; Clinical chemistry; Klinisk kemi;

    Abstract : An increase in the plasma level of creatine kinase MB (CKMB) has long been used to indicate acute myocardial infarction (AMI). For optimal treatment an early diagnosis is important. An increase in the ratio between the two isoforms of CKMB is seen much earlier than an increase in the total level of CKMB. READ MORE

  5. 5. Fast isolation of proteins from bioreactors using affinity chromatography techniques

    University dissertation from Eva Pålsson, Tillämpad biokemi, Lunds universitet

    Author : Eva Pålsson; Lund University.; Lunds universitet.; [2000]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; chromatography; protein purification; analyses; dispersion; chromatography matrices; Biochemistry; Biokemi; Metabolism; expanded bed; metabolism; Biotechnology; Bioteknik;

    Abstract : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. READ MORE