Search for dissertations about: "Affinity Capillary Electrophoresis"

Showing result 1 - 5 of 11 swedish dissertations containing the words Affinity Capillary Electrophoresis.

2. 1. Method development for affinity capillary electrophoresis of ß2-glycoprotein I and biological ligands

   Author : Maria E. Bohlin; Lars G Blomberg; Niels H H Heegaard; Jan Leo Reubsaet; Karlstads universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Capillary Electrophoresis; β2-glycoprotein I; acidic pretreatment; pH hysteresis effect; Affinity Capillary Electrophoresis; Kemi; Chemistry;

   Abstract : The final goal of this study is to establish a microscale analysis method that allows solution phase characterization of interactions between β2-glycoprotein I (β2gpI) and some of its ligands. Human β2gpI is a phospholipid- and heparin-binding plasma glycoprotein. READ MORE

4. 2. Enantiomeric separation by capillary electrophoresis : With special emphasis on the partial filling technique

   Author : Ahmad Amini; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical chemistry; Farmaceutisk kemi; Pharmaceutical chemistry; Farmaceutisk kemi; analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry;

   Abstract : Capillary electrophoresis as a powerful separation technique was employed for resolution of racemic drugs. Taurodeoxycholate (TDC), different cyclodextrins and α1- acid glycoprotein (AGP) were dissolved in the background electrolyte at low pH (3.0 to 4.0 ) as chiral selectors. READ MORE

5. 3. Molecularly Imprinted Matrices for Electrochromatography

   Author : Leif Schweitz; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chemical technology and engineering; capillary electrophoresis; enantiomer; film coating; microparticle; continuous bed; monolith; molecular imprinting; capillary electrochromatography; Kemiteknik och kemisk teknologi; Analytical chemistry; Analytisk kemi;

   Abstract : This thesis deals with the combination of electrochromatography and molecular imprinting technology. Electrochromatography is characterised by high separation efficiency, simple instrumentation, and minute consumption of sample. READ MORE

6. 4. Enantioselective Binding of Designed Propranolol Analogues to the Cellobiohydrolase Cel7A

   Author : Alexandra Fagerström; Centrum för analys och syntes; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Strukturkemi; Structure chemistry; enzymologi; Proteiner; enzymology; Proteins; Naturvetenskap; Natural science; capillary electrophoresis; chiral recognition; enantiomeric selectivity; cellobiohydrolase Cel7A; propranolol; Organic chemistry; Organisk kemi;

   Abstract : This thesis describes the affinity and enantiomeric selectivity of the cellobiohydrolase Cel7A (isolated from Hypocrea jercorina). The chiral recognition of the enzyme has been studied with respect to amino alcohols and new ligands have been designed, based on the structure of the common ?-blocker propranolol. READ MORE

7. 5. Quantitative Bioanalysis : Liquid separations coupled to targeted mass spectrometric measurements of bioactive compounds

   Author : Björn Arvidsson; Jonas Bergquist; Magnus Wetterhall; Gérard Hopfgartner; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Method development; Enhanced microdialysis; Column switching; Liquid chromatography; Capillary electrophoresis; Electrospray ionization; Mass spectrometry; Triple quadrupole; Time-of-flight; Quantitation; Validation; Analytisk kemi;

   Abstract : Performing quantitative analysis of targeted bioactive compounds in biological samples, such as blood plasma, cerebrospinal fluid or extracts from pig liver, put high demands on the ruggedness of the method acquiring the results. In addition to the complexity of the sample matrix, the bioactive compounds targeted for analysis usually have low levels of natural abundance, further increasing the demand on the analytical method sensitivity. READ MORE