Search for dissertations about: "Antibody validation"

Showing result 11 - 15 of 34 swedish dissertations containing the words Antibody validation.

  2. 11. Databases for antibody-based proteomics

    Author : Erik Björling; Mathias Uhlén; Alvis Brazma; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteomics; antibodies; database; biomarker; website; Bioengineering; Bioteknik;

    Abstract : Humans are believed to have ~20,500 protein-coding genes andmuch effort has over the last years been put into the characterizationand localization of the encoded proteins in order to understand theirfunctions. One such effort is the Human Proteome Resource (HPR)project, started in Sweden 2003 with the aim to generate specificantibodies to each human protein and to use those antibodies toanalyze the human proteome by screening human tissues and cells. READ MORE

  4. 12. High-throughput protein analysis using mass spectrometry-based methods

    Author : Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Keywords : Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Abstract : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. READ MORE

  5. 13. Development of novel affinity enrichment strategies for clinical applications using selected reaction monitoring

    Author : Andreas Hober; Mathias Uhlén; Fredrik Edfors; Björn Forsström; Johan Malmström; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; proteomics; mass spectrometry; selected reaction monitoring; absolute quantification; antibody validation; precision medicine; Biotechnology; Bioteknologi;

    Abstract : Proteins are key components of any living organism and an essential part of life itself. They can provide cells with structure and perform life-sustaining intracellular reactions. As organisms grow more complex, this task expands even further. READ MORE

  6. 14. Antibody-based bead arrays for high-throughput protein profiling in human plasma and serum

    Author : Kimi Drobin; Jochen M. Schwenk; Peter Nilsson; Ulrika Qundos; Angela Silveira; Maria Pernemalm; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; affinity proteomics; immunoassay; antibody microarray; suspension bead array; protein profiling; plasma; serum; biomarker discovery; osteoporosis; inflammatory bowel disease; Crohn s disease; ulcerative colitis; Medical Technology; Medicinsk teknologi;

    Abstract : Affinity-based proteomics utilizes affinity binders to detect target proteins in a large-scale manner. This thesis describes a high-throughput method, which enables the search for biomarker candidates in human plasma and serum. READ MORE

  7. 15. At the intersection of proteomics and pathology : application of mass spectrometry-based protein quantification to histopathology and antibody validation

    Author : Fabio Socciarelli; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The paradigm of Precision Medicine relies on the molecular stratification of patients to select “the right patient for the right drug”. The genomic approach has tried to answer this question, but large genomic studies have raised more questions than given answers. READ MORE