Search for dissertations about: "Antibody validation"

Showing result 16 - 20 of 34 swedish dissertations containing the words Antibody validation.

  2. 16. Recombinant Antibodies – Tools for building proteomic platforms

    Author : Anna Säll; Institutionen för immunteknologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Antibody development; recombinant scFv antibodies; phage display; affinity proteomics; mass spectrometry;

    Abstract : This thesis, which is based on four original papers, covers different aspects of the evolvement and development of proteomic platforms and the construction of human synthetic antibody libraries for the isolation of antibody reagents has been very central. There is an accelerating need for technologies capable of providing proteomic analysis of high sensitivity with good accuracy and an overall broad coverage as well as for biomarker validation. READ MORE

  4. 17. Affinity assays for profiling disease-associated proteins in human plasma

    Author : Sanna Byström; Jochen M. Schwenk; Claudia Fredolini; Peter Nilsson; Jennifer Van Eyk; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; affinity proteomics; immunoassay; antibody microarray; suspension bead array; protein profiling; immuno-capture; plasma; serum; cerebrospinal fluid; biomarker discovery; multiple sclerosis; malignant melanoma; mammographic breast density; Bioteknologi; Biotechnology;

    Abstract : Affinity-based proteomics offers opportunities for the discovery and validation of disease-associated proteins in human body fluids. This thesis describes the use of antibody-based immunoassays for multiplexed analysis of proteins in human plasma, serum and cerebrospinal fluid (CSF). READ MORE

  5. 18. Tools for the development of intensified perfusion processes for mammalian cell culture

    Author : Hubert Schwarz; Véronique Chotteau; Mark Smales; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinant protein biomanufacturing; Perfusion process; Integrated continuous bioprocessing; Monoclonal antibody; Erythropoietin; Chinese Hamster Ovary cells; Human Embryonic Kidney cells; Perfusion medium optimization; Cell specific perfusion rate; Raman spectroscopy; N-glycosylation; Microbioreactors; Design of Experiments; Biotechnology; Bioteknologi;

    Abstract : Recombinant protein therapeutics have become an indispensable part of modern medicine to treat a wide variety of diseases. Their manufacture is mostly based on mammalian cells with fed-batch production bioreactors and downstream batch purification unit operations. READ MORE

  6. 19. Human herpesvirus 6A and 6B : assay validation, virus-host interaction and clinical relevance

    Author : Elin Engdahl; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Human herpesvirus (HHV)-6A and HHV-6B are two ubiquitous herpesviruses that after primary infection can stay latent in the host. The two viruses can reactivate from latency and cause secondary complications. Also, diseases like multiple sclerosis and epilepsy have been associated with HHV-6A and HHV-6B. READ MORE

  7. 20. Health-related quality of life, treatment satisfaction and clinical aspects of patients with primary antibody deficiency receiving subcutaneous IgG self-infusions at home

    Author : Uwe Nicolay; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Patients with primary antibody deficiencies (PAD) are unable to produce sufficient amounts of antibodies and are therefore susceptible to severe bacterial infections. The standard therapy has been intravenous infusions of immunoglobulin G (IVIG) every 2-4 weeks. READ MORE