Search for dissertations about: "Aqueous two-phase systems"

Showing result 11 - 15 of 27 swedish dissertations containing the words Aqueous two-phase systems.

  2. 11. Selective extraction systems for detection and purification of membrane proteins

    Author : Ulf Sivars; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Abstract : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. READ MORE

  4. 12. PCR-based detection of microorganisms in complex biological samples

    Author : Pär-G Lantz; Teknisk mikrobiologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Density gradient centrifugation; DNA extraction; Aqueous two-phase systems; Meat; Faeces; Cheese; Yeast; Helicobacter pylori; Yersinia enterocolitica; Listeria monocytogenes; PCR; Inhibition; Sample preparation; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : The detection of microorganisms by PCR can be divided into four steps: (1) sample collection, (2) sample preparation, (3) DNA amplification and (4) detection of PCR products. The major problem in developing PCR-based detection methods is the sample preparation step. READ MORE

  5. 13. Hydrophobic Fusion Tags: Implications For Bioseparation and Cellular Expression

    Author : Anna Collén; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein production; secretion; aqueous two-phase systems; Trichoderma reesei; endoglucanase; Biochemistry; Metabolism; Biokemi; metabolism;

    Abstract : The studies in this thesis have shown that the partitioning of endoglucanase I (EGI, Cel7B) from T. reesei could be significantly improved by relatively minor genetic engineering. By adding short peptides composed of tryptophan and proline residues to EGI, extreme partitioning could be obtained. READ MORE

  6. 14. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Author : Sheryl Fernandes; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Abstract : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. READ MORE

  7. 15. A radical view of haemoglobin - protein engineering and characterisation

    Author : Marie Grey; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

    Abstract : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. READ MORE