Search for dissertations about: "Aqueous two-phase systems"

Showing result 6 - 10 of 27 swedish dissertations containing the words Aqueous two-phase systems.

  2. 6. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Author : Cynthia Hassinen; KTH; []
    Keywords : E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Abstract : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. READ MORE

  4. 7. Separation Methods in Membrane Proteomics -Integration of Aqueous Two-Phase Partitioning for Enrichment and Pre-Fractionation

    Author : Henrik Everberg; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteins; metabolism; Biokemi; Metabolism; Biochemistry; Aqueous two-phase partitioning; enzymology; Proteiner; enzymologi; Proteomics;

    Abstract : The study of the protein complement of a genome is called proteomics. Proteomics can be defined as identification, quantification and characterization of a total set of proteins expressed at different time-points or under different cellular states to provide global information about biological processes. READ MORE

  5. 8. Interfacial Phenomena in Two-Phase systems: Emulsions and slag Foaming

    Author : Abha Kapilashrami; Seshadri Seetharaman; Bridget Harris; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; film formation; o w emulsion; substrate effect; slag forming; Materialvetenskap; Materials science; Teknisk materialvetenskap;

    Abstract : In the present work studies were performed to provide understanding for further model development of the two-phase phenomena, film formation from o/w emulsions and slag foaming.The drying of o/w emulsions of different oil viscosities on hydrophobic and hydrophilic substrates was studied. READ MORE

  6. 9. Studies on rat and human lymphocytes separated in aqueous two-phase systems

    Author : Per Malmström; Lund Wallenberglaboratoriet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Abstract : .... READ MORE

  7. 10. Protein-Polymer Interaction and Association in Aqueous Solution

    Author : Malin Jönsson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; Fysikalisk kemi; Makromolekylär kemi; Macromolecular chemistry; enzymologi; Proteiner; enzymology; Proteins; Aqueous two-phase systems; Monte Carlo Simulations; hydrophobic effect; surface hydrophobicity; plasmid DNA; Protein; polymer;

    Abstract : The aim of this thesis has been to study protein-polymer interaction and association. Two different techniques were used; Monte Carlo simulations were combined with partitioning in polymeric aqueous two-phase systems. Monte Carlo simulations have been used to evaluate the hydrophobic interaction in electrolyte solution. READ MORE