Search for dissertations about: "Bacterial Display"

Showing result 1 - 5 of 69 swedish dissertations containing the words Bacterial Display.

  2. 1. Bacterial display systems for engineering of affinity proteins

    Author : Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
    Keywords : Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

    Abstract : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. READ MORE

  4. 2. Towards Anti-Virulence Antimicrobials, Discovery and Development of Sortase A Inhibitors and Investigations of Bacterial Phenotypes

    Author : Patrick M. Wehrli; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Sortase A; SrtA; Inhibitors; Anti-virulence; Bacterial analysis; FTIR spectroscopy; Bacterial phenotyping; Design of Experiment; Multivariate Data Analysis; PCA; ToF-SIMS; Time-of-flight secondary-ion-mass-spectrometry; Lipid analysis; ppGpp; Stringent response;

    Abstract : Antibiotic resistance is an emerging and serious threat to public health. Immediate actions are required to preserve current antibiotics while intensifying research efforts towards the development of new effective therapeutics. READ MORE

  5. 3. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Author : Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

    Abstract : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. READ MORE

  6. 4. Functional studies and engineering of family 1 carbohydrate-binding modules

    Author : Janne Lehtiö; KTH; []
    Keywords : Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Abstract : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. READ MORE

  7. 5. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies

    Author : Ann-Christin Malmborg Hager; Institutionen för immunteknologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; affinity; antibody; kinetic; Immunologi; serologi; serology; Immunology; Bioteknik; Biotechnology; library; selection; surface display; phage display; association rate; dissociation rate;

    Abstract : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. READ MORE