Search for dissertations about: "Biomembrane"

Showing result 1 - 5 of 16 swedish dissertations containing the word Biomembrane.

  1. 1. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Christine Lagerquist Hägglund; Uppsala universitet.; [2003]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Affinity; Aromatic amino acids; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dihydrocytochalasin B; Dissociation constant; Drug absorption; Ethanol; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Specific; Streptavidin; Tyrosine; Tryptophan; Biokemi; MEDICINE Chemistry Biochemistry; MEDICIN Kemi Biokemi;

    Abstract : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. READ MORE

  2. 2. Immobilized biomembrane chromatographic analyses : with reference to membrane protein purification and reconstitution

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Eggert Brekkan; Lars Haneskog; Uppsala universitet.; Uppsala universitet.; [1997]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; NATURVETENSKAP;

    Abstract : .... READ MORE

  3. 3. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Ingo Gottschalk; Uppsala universitet.; [2002]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Affinity; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dissociation constant; Drug absorption; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Solute; Specific; Streptavidin; Wheat germ agglutinin; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi; Biokemi; Biochemistry;

    Abstract : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. READ MORE

  4. 4. Spectroscopic studies of dynorphin neuropeptides and the amyloid beta-peptide The consequences of biomembrane interactions

    University dissertation from Stockholm : Institutionen för biokemi och biofysik

    Author : Loïc Hugonin; Stockholms universitet.; [2007]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; TECHNOLOGY Engineering physics Biophysics; TEKNIKVETENSKAP Teknisk fysik Biofysik; Biophysics; biofysik;

    Abstract : Dynorphin A, dynorphin B and big dynorphin are endogenous opioid neuropeptides. They play an important role in a wide variety of physiological functions such as regulation of pain processing and memory acquisition. Such actions are generally mediated through the κ-receptors. READ MORE

  5. 5. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Shusheng Zuo; Uppsala universitet.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Cholesterol; Cytochalasin B; Glucose; GLUT1; Hummel and Dreyer analysis; Immobilization; PEG 5000 -DSPE; Biomembrane; Proteoliposome; Quantitative frontal affinity chromatography; Red blood cell streptavidin-biotin immobilization; Sulfhydryl affinity chromatography; The modified micro-Bradford CaPE assay; MALDI-ToF-MS; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi;

    Abstract : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. READ MORE