Search for dissertations about: "Blotting"

Showing result 1 - 5 of 116 swedish dissertations containing the word Blotting.

  2. 1. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

    Author : Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Abstract : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. READ MORE

  4. 2. Expression and Regulation of P2 Receptors in Human Circulation

    Author : Lingwei Wang; Sektion II; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Western blotting; Cardiovascular system; Kardiovaskulära systemet; vascular remodeling; systemic lupus erythematosus; smooth muscle cell; shear stress; real-time PCR; platelets; P2 receptor; gene expression; erythrocyte; ATP release; endothelium;

    Abstract : P2 receptors mediate the actions of the extracellular nucleotides ATP, ADP, UTP and UDP regulating several physiological responses including cardiac function, vascular tone, smooth muscle cell (SMC) proliferation, platelet aggregation, and the release of endothelial factors. The aim of the present study was to investigate the mRNA and protein expression of P2 receptors in human SMCs, endothelial cells (ECs), erythrocytes and platelets, the regulation of P2 receptors under shear stress in human vessels, the changes of platelet P2 receptors in patients with systemic lupus erythematosus (SLE) and the regulation of plasma ATP levels by P2 receptor on erythrocytes. READ MORE

  5. 3. Uveal melanoma : cytogenetics, molecular biology and tumor immunology

    Author : Charlotta All-Ericsson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Uveal melanoma; prognostic markers; treatment; CGH; chromosomal aberrations; HLA; IHC; Western blotting; IGF-1R; c-kit; STT571;

    Abstract : Uveal melanoma is the most common primary intraocular malignancy. The metastatic spread is hematogenous and exclusively to the liver. Although eye-sparing treatments are used more frequently the tumor-related mortality in uveal melanoma is still 30-50%. Only 2% of the patients have detectable metastases at the time of diagnosis. READ MORE

  6. 4. Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor

    Author : Hanna Wallin; Avdelningen för klinisk kemi och farmakologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cathepsin; cell line; co-localisation; cystatin C; internalisation; legumain;

    Abstract : Cystatin C is a cysteine protease inhibitor, aimed for secretion, as it is produced with a signal peptide. Its target enzymes are thought to be the lysosomal cysteine cathepsins and legumain. READ MORE

  7. 5. Characterization of novel podocyte proteins in the glomerular filtration barrier

    Author : Laleh Sistani; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The kidney is considered the most important organ of excretion eliminating the body from metabolic waste products. The nephron is the functional unit of the kidney, composed of a cluster of capillaries named glomerulus, and a tubular system. The blood is filtered in the glomerular capillaries across the glomerular filtration barrier. READ MORE