Search for dissertations about: "CRISPR Cas9"

Showing result 1 - 5 of 62 swedish dissertations containing the words CRISPR Cas9.

  2. 1. Modeling Human Hematopoiesis Using the CRISPR/Cas9 System

    Author : Alexandra Bäckström; Avdelningen för molekylärmedicin och genterapi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hematopoiesis; Hematopoietic stem and progenitor cells; HSC regulation; CRISPR Cas9; Gene editing;

    Abstract : Hematopoietic stem cells (HSCs) have the ability to self-renew and to give rise to all blood cells of the different lineages, and are thereby responsible for the replenishment of blood cells throughout life. These cells are tightly regulated by extrinsic and intrinsic regulators, such as signals from the bone marrow microenvironment, complex transcription factor networks and epigenetic regulators. READ MORE

  4. 2. Multifaceted RNA-mediated regulatory mechanisms in Streptococcus pyogenes

    Author : Anaïs Le Rhun; Emmanuelle Charpentier; Franz Narberhaus; Umeå universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Streptococcus pyogenes; small RNAs; CRISPR; Cas9; tracrRNA; RNases; gene expression; RNA sequencing; Infectious Diseases; infektionssjukdomar; mikrobiologi; Microbiology;

    Abstract : Bacterial pathogens rely on precise regulation of gene expression to coordinate host infection processes and resist invasion by mobile genetic elements. An interconnected network of protein and RNA regulators dynamically controls the expression of virulence factors using a variety of mechanisms. READ MORE

  5. 3. Advancing CRISPR technologies to engineer yeast metabolism

    Author : Raphael Ferreira; Chalmers University of Technology; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; oleochemicals; metabolic engineering; Saccharomyces cerevisiae; CRISPR;

    Abstract : The advent of genetic engineering tools has initiated an era of manipulating microorganisms for the production of valuable compounds for our society. Precise engineering of these microbes commonly requires introducing genetic modifications such as gene deletion, overexpression, and accurate regulation in order to enhance the production of the compound of interest. READ MORE

  6. 4. CRISPR/Cas9-based therapies and the role of astrocytes in Alzheimer’s disease and Parkinson’s disease

    Author : Evangelos Konstantinidis; Martin Ingelsson; Anna Erlandsson; Vilmantas Giedraitis; Magnus Essand; Harald Steiner; Uppsala universitet; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alzheimer s disease; Parkinson s disease; gene editing; CRISPR Cas9; amyloid-beta; astrocytes; neurons; iPSCs; electrophysiology;

    Abstract : Alzheimer’s disease (AD) and Parkinson’s disease (PD) are the two most common neurodegenerative disorders. Whereas the AD brain features plaques of amyloid-beta (Aβ) and neurofibrillary tangles of tau, the PD brain is characterized by Lewy bodies and Lewy neurites containing α-synuclein (αSyn). READ MORE

  7. 5. CRISPR based technologies for high-throughput metabolic engineering of yeast

    Author : Christos Skrekas; Chalmers University of Technology; []
    Keywords : screening; enrichment; CRISPR; metabolic engineering; cloning; Saccharomyces cerevisiae; selection; directed evolution; library;

    Abstract : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. READ MORE