Search for dissertations about: "CRISPR Cas9"

Showing result 21 - 25 of 78 swedish dissertations containing the words CRISPR Cas9.

  2. 21. Functional characterization of the biological significance of the ZBED6/ZC3H11A locus in placental mammals

    Author : Shady Younis; Leif Andersson; Ola Hansson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ZBED6; IGF2; ZC3H11A; Muscle development; Transcriptome analysis; CRISPR Cas9; mRNA export; Molekylär genetik; Molecular Genetics; Bioinformatik; Bioinformatics;

    Abstract : The recent advances in molecular and computational biology have made possible the study of complicated transcriptional regulatory networks that control a wide range of biological processes and phenotypic traits. In this thesis, several approaches were combined including next generation sequencing, gene expression profiling, chromatin and RNA immunoprecipitation, bioinformatics and genome editing methods in order to characterize the biological significance of the ZBED6 and ZC3H11A genes. READ MORE

  4. 22. Identification and Functional Significance of Aberrant Long Non-coding RNAs in Acute Myeloid Leukemia : Biological and Prognostic Implications

    Author : Anne H. Neddermeyer; Sören Lehmann; Jonas Larsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; acute myeloid leukemia AML ; hematopoiesis; long non-coding RNAs lncRNAs ; RNA- and CAGE-sequencing; CRISPRi; CRISPR-Cas9; NEAT1; APL; NPM1; IDH2; all-trans retinoic acid; arsenic trioxide; venetoclax; drug resistance and sensitivity; prognosis; RADICL-sequencing; Molekylär medicin; Molecular Medicine;

    Abstract : Acute myeloid leukemia (AML) is the most frequently diagnosed type of acute leukemia in adults. It commonly affects people aged 60 or older, as incidence increases with age, and it is characterized by the accumulation of immature hematopoietic progenitor cells in the bone marrow. READ MORE

  5. 23. Engineering yeast for improved recombinant protein production

    Author : Yanyan Wang; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; point mutation; genome-scale modeling; recombinant protein; omics analysis; CRISPR Cas9; protein secretion; Saccharomyces cerevisiae;

    Abstract : Recombinant proteins are broadly used from basic research to therapeutic development and include industrial enzymes and pharmaceutical proteins. The increasing demand for improved production and enhanced quality of recombinant proteins requires robust biotech-based strategies to overcome the limitations of protein extraction from natural sources. READ MORE

  6. 24. A molecular exploration of sensory responses in c. elegans

    Author : Longjun Pu; Changchun Chen; Simon Tuck; Sara I Wilson; Zhiyong Shao; Umeå universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; C. elegans; sensation; CRISPR Cas9; G protein-coupled receptors; neuropeptides; acute hypoxia; pathogen; chemosensation; hydrogen sulfide; HIF-1; iron;

    Abstract : Sensation provides a pivotal ability, allowing animals to survive in complex environments. The cues sensed by animals are represented by external stimuli and internal signals. However, the mechanisms mediating sensations in molecular and cellular level are still not well-studied. In this thesis, by using free-living nematodes C. READ MORE

  7. 25. Functional genomics approaches to dissect causal DNA variations in obesity and type 2 diabetes

    Author : Mi Huang; Genetisk och molekylär epidemiologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; GWAS; Obesity; Type 2 diabetes; CRISPR Cas9; precision medicine; adipocytes; functional genomics;

    Abstract : GWAS studies have identified hundreds of loci that associate with obesity and type 2 diabetes. However, the functional relevance of the genetic variants, and their impact on disease or traits, is largely unknown. READ MORE