Search for dissertations about: "CRISPR Cas9"

Showing result 6 - 10 of 79 swedish dissertations containing the words CRISPR Cas9.

2. 6. CRISPR based technologies for high-throughput metabolic engineering of yeast

   Author : Christos Skrekas; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cloning; Saccharomyces cerevisiae; enrichment; metabolic engineering; selection; library; screening; CRISPR; directed evolution;

   Abstract : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. READ MORE

4. 7. CRISPR Screens Identify Candidate Therapeutic Targets in Leukemia

   Author : Maria Rodriguez Zabala; Avdelningen för klinisk genetik; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Acute myeloid leukemia; CRISPR screen; leukemia stem cell; cell surface receptor; metabolism; immunotherapy; CXCR4; GLUT1; TFR1; H2-K1;

   Abstract : Acute myeloid leukemia (AML) is a complex hematological malignancy marked by proliferation of immature myeloid cells with a dismal 5-year survival. A major challenge is the persistence of leukemia stem cells (LSCs) after standard treatment, leading to relapse. READ MORE

5. 8. Exploring early development and regenerative medicine using CRISPR/Cas9

   Author : Nerges Winblad; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : With their intrinsic capacity to self-renew and their potential to differentiate to specialized tissues, human embryonic stem cells (hESCs) have many applications in the fields of cell therapy and developmental biology. By applying clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) genome engineering on selected genes in hESCs, we are able to create cells with desired characteristics. READ MORE

6. 9. Targeted Long-read Sequencing : Development and Applications in Medical Genetics

   Author : Ida Höijer; Ulf B. Gyllensten; Adam Ameur; Lars Feuk; Winston Timp; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; long-read sequencing; targeted sequencing; targeted enrichment; CRISPR-Cas9; off-targets; medical genetics; repeat expansion; structural variant;

   Abstract : Targeted sequencing has the advantage of providing pinpointed DNA information, while costs and data-analysis efforts are reduced. If targeted sequencing is combined with single molecule long-read sequencing, it can become a powerful tool to investigate genomic regions traditionally difficult using the predominantly used short-read sequencing platforms, including repetitive regions and large structural variants. READ MORE

7. 10. From GWAS to gene : Zebrafish as a model system for large-scale studies of obesity and comorbidities

   Author : Eugenia Mazzaferro; Marcel den Hoed; Randall Peterson; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; zebrafish; genetic screens; gene editing; CRISPR Cas9; obesity; Medicinsk genetik; Medical Genetics;

   Abstract : Obesity increases the risk of developing comorbidities such as cardiovascular and metabolic diseases. However, not all obese individuals develop comorbidities, and those that do not are referred to as “metabolically healthy obesity” individuals. READ MORE