Search for dissertations about: "CRISPR"

Showing result 1 - 5 of 26 swedish dissertations containing the word CRISPR.

  1. 1. The type I-E CRISPR-Cas system Biology and applications of an adaptive immune system in bacteria

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Lina Amlinger; Uppsala universitet.; [2017]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; CRISPR; CRISPR-Cas; virus defense; bacteria; bacteriophage; adaptation; spacer integration; interference; gene silencing; fluorescent reporter; Escherichia coli; Mikrobiologi; Microbiology;

    Abstract : CRISPR-Cas systems are adaptive immune systems in bacteria and archaea, consisting of a clustered regularly interspaced short palindromic repeats (CRISPR) array and CRISPR associated (Cas) proteins. In this work, the type I-E CRISPR-Cas system of Escherichia coli was studied.CRISPR-Cas immunity is divided into three stages. READ MORE

  2. 2. Advancing CRISPR technologies to engineer yeast metabolism

    University dissertation from Gothenburg : Chalmers tekniska högskola

    Author : Raphael Ferreira; [2019]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; oleochemicals; metabolic engineering; Saccharomyces cerevisiae; CRISPR;

    Abstract : The advent of genetic engineering tools has initiated an era of manipulating microorganisms for the production of valuable compounds for our society. Precise engineering of these microbes commonly requires introducing genetic modifications such as gene deletion, overexpression, and accurate regulation in order to enhance the production of the compound of interest. READ MORE

  3. 3. Multifaceted RNA-mediated regulatory mechanisms in Streptococcus pyogenes

    University dissertation from Umeå : Umeå university

    Author : Anaïs Le Rhun; Umeå universitet.; Umeå universitet.; Umeå universitet.; [2015]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Streptococcus pyogenes; small RNAs; CRISPR; Cas9; tracrRNA; RNases; gene expression; RNA sequencing; Infectious Diseases; infektionssjukdomar; mikrobiologi; Microbiology;

    Abstract : Bacterial pathogens rely on precise regulation of gene expression to coordinate host infection processes and resist invasion by mobile genetic elements. An interconnected network of protein and RNA regulators dynamically controls the expression of virulence factors using a variety of mechanisms. READ MORE

  4. 4. Small RNAs, Big Consequences Post-transcriptional Regulation and Adaptive Immunity in Bacteria

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Mirthe Hoekzema; Uppsala universitet.; [2018]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; small RNA; sRNA; non-coding RNA; Hfq; gene regulation; post-transcriptional regulation; biofilm; OmrA; OmrB; toxin-antitoxin; TisB; IstR-1; Persisters; CRISPR-Cas; CRISPR-Cas adaptation reporter; Escherichia coli; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

    Abstract : It is nowadays widely accepted that non-coding RNAs play important roles in post-transcriptional regulation of genes in all kingdoms of life. In bacteria, the largest group of RNA regulators are the small RNAs (sRNAs). READ MORE

  5. 5. Optical Mapping of Bacterial Plasmids

    University dissertation from Gothenburg : Chalmers tekniska högskola

    Author : Vilhelm Müller; [2017]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; competitive binding; optical mapping; fluorescence microscopy; DNA; plasmids; CRISPR Cas9; antibiotic resistance; nanofluidics; single-molecule;

    Abstract : Bacteria that have acquired resistance to antibiotics represent one of the largest threats to human health and modern health care. The genes encoding resistance are frequently spread via the transfer of plasmids, which are circular double stranded DNA molecules separate from the chromosomal DNA of the bacteria. READ MORE