Search for dissertations about: "CRISPR"

Showing result 6 - 10 of 98 swedish dissertations containing the word CRISPR.

  2. 6. CRISPR-based drug target discovery : implications for CRISPR-based therapies

    Author : Long Jiang; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The CRISPR system was discovered in prokaryotic cells, and it is now one of the most efficient molecular tools to modify genes in eukaryotic cells. It is widely used to inactivate or modify genes in cell lines, animal models, and is also used in several clinical gene therapy trials. READ MORE

  4. 7. Multifaceted RNA-mediated regulatory mechanisms in Streptococcus pyogenes

    Author : Anaïs Le Rhun; Emmanuelle Charpentier; Franz Narberhaus; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Streptococcus pyogenes; small RNAs; CRISPR; Cas9; tracrRNA; RNases; gene expression; RNA sequencing; Infectious Diseases; infektionssjukdomar; mikrobiologi; Microbiology;

    Abstract : Bacterial pathogens rely on precise regulation of gene expression to coordinate host infection processes and resist invasion by mobile genetic elements. An interconnected network of protein and RNA regulators dynamically controls the expression of virulence factors using a variety of mechanisms. READ MORE

  5. 8. Functional Screens Identify Vulnerabilities in Acute Leukemia

    Author : Ramprasad Ramakrishnan; Avdelningen för klinisk genetik; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Acute leukemia; CRISPR; CXCR4; CXCL12; TNFSF13; DUX4; Interleukin 4; leukemia stem cell; macrophage;

    Abstract : Acute leukemia refers to a group of aggressive hematological malignancies of myeloid and lymphoid lineages termed acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) respectively. Acute leukemia is characterized by the presence of underlying genetic aberrations which alter the biology of normal hematopoietic cells resulting in the accumulation of immature abnormally differentiated blast cells. READ MORE

  6. 9. Deregulated epigenetics and cancer stem cells in brain tumours

    Author : Anna Wenger; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cancer stem cell; glioblastoma; paediatric; methylation; heterogeneity; CRISPR;

    Abstract : Glioblastoma (GBM) is an incurable brain tumour with dismal prognosis as the median survival for afflicted patients is only 8 months. Aberrations of epigenetic processes, which govern gene expression, are involved in cancer, but these epigenetic mechanisms are reversible and can potentially be corrected by treatment. READ MORE

  7. 10. Improving integration efficiency and precision of CRISPR/Cas9-mediated genome editing

    Author : Sandra Wimberger; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Genome editing; CRISPR-Cas9; DNA repair; genomic integration; off-targets;

    Abstract : The clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR-associated protein 9 (Cas9) system has revolutionized the field of genome engineering, providing a cost-effective and fast tool for targeted gene modifications. Endogenous repair pathways, including error-prone non-homologous end joining and alternative end joining, or precise homology-directed repair (HDR), mend Cas9-induced DNA double-strand breaks. READ MORE