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Showing result 1 - 5 of 35 swedish dissertations matching the above criteria.

  2. 1. Studies of individual pancreatic -cells : Electrophysiological analysis of rhythmic behaviour and development of new techniques

    Author : Staffan Dryselius; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; Diabetes; pancreatic beta-cells; insulin release; signal transduction; cytoplasmic calcium; oscillations; patch clamp; ATP-regulated potassium channels; voltage-dependent calcium channels; metabolism; calcium ion; strontium ion; manganese ion; wavelets; de-noising; pattern recognition; superfusion; solution changes; microscopic labelling; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

    Abstract : The insulin concentration in blood varies periodically, which is believed to prevent down-regulation of the hormone receptors. Loss of the regular insulin oscillations is considered to be an early sign of developing diabetes. READ MORE

  4. 2. The interplay of ions in the stimulation of the pancreatic -cell

    Author : Michael Eberhardson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; diabetes; pancreatic β-cells; cytoplasmic calcium; oscillations; glucose; tetrodotoxin; tolbutamide; veratridine; tetraethylammonium; quinine; cesium; H2DIDS; furosemide; transients; microfluorometry; fura-2; MQAE; calcium channels; potassium channels; sodium channels; chloride channels; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

    Abstract : Glucose stimulation of insulin release is mediated by depolarisation of the pancreatic β-cells with accompanying entry of Ca2+ through voltage-dependent channels. An important feature of the glucose-induced depolarisation is its rhythmicity causing oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i), which trigger pulsatile release of insulin. READ MORE

  5. 3. Calcium transporting ATPases in plant cells

    Author : Malin Olbe; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Triticum aestivum; calcium; Brij 58; calmodulin; Ca2 signalling; inside-out; endoplasmic reticulum; plant; plasma membrane; proteolysis; P-type ATPase; spinach; Spinacea oleracea; trypsin; two-phase; wheat.; Plant biochemistry; Växtbiokemi;

    Abstract : Plant cells carry Ca2+ ATPases to maintain the low resting level of cytosolic Ca2+ (0.1-0.2 µM) found in all eukaryotes. Plant Ca2+ ATPases are mainly located in the plasma membrane, the endoplasmic reticulum and in the vacuolar membrane, and consequently large Ca2+ gradients are created across these membranes. READ MORE

  6. 4. Pulsatile insulin release from single islets of Langerhans

    Author : Johanna Westerlund; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell biology; islets of Langerhans; insulin; ELISA; cytoplasmic calcium; oscillations; type 2 diabetes; glucose; diazoxide; potassium; tolbutamide; DNP; antimycin A; iodoacetamide; microfluorometry; fura-2; KATP channels; Cellbiologi; Cell biology; Cellbiologi; medicinsk cellbiologi; Medical Cell Biology;

    Abstract : Insulin release from single islets of Langerhans is pulsatile. The secretory activities of the islets in the pancreas are coordinated resulting in plasma insulin oscillations. Nutrients amplitude-regulate the insulin pulses without influencing their frequency. READ MORE

  7. 5. On the Generation of cAMP Oscillations and Regulation of the Ca2+ Store-operated Pathway in Pancreatic Islet α- and β-cells

    Author : Geng Tian; Erik Gylfe; Anders Tengholm; Patrick Gilon; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cAMP; oscillations; adrenaline; GLP-1; phosphodiesterase; palmitate; STIM1; Orai1; store-operated calcium entry; insulin secretion; glucagon secretion; β-cell; α-cell; Medical Cell Biology; Medicinsk cellbiologi;

    Abstract : Insulin and glucagon are released in pulses from pancreatic β- and α-cells, respectively. Both cell types are electrically excitable, and elevation of the cytoplasmic Ca2+ concentration ([Ca2+]i) due to depolarization with voltage-dependent entry of the cation is the main trigger of hormone secretion. READ MORE