Search for dissertations about: "Candida antarctica lipase B in pichia pastoris by mutagenesis"

Found 2 swedish dissertations containing the words Candida antarctica lipase B in pichia pastoris by mutagenesis.

2. 1. Expression and Mutagenesis studies of Candida antactica lipase B

   Author : Johanna C. Rotticci-Mulder; KTH; []
   Keywords : Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

   Abstract : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. READ MORE

4. 2. Exploiting enzyme promiscuity for rational design

   Author : Cecilia Branneby; Karl Hult; Alex Zaks; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; alanine racemase; substrate specificity; reaction specificity; enzyme catalytic promiscuity; Biokemi; Biochemistry; Biokemi;

   Abstract : Enzymes are today well recognized in various industrial applications, being an important component in detergents, and catalysts in the production of agrochemicals, foods, pharmaceuticals, and fine chemicals. Their large use is mainly due to their high selectivity and environmental advantage, compared to traditional catalysts. READ MORE