Search for dissertations about: "DNA barcoding"

Showing result 1 - 5 of 13 swedish dissertations containing the words DNA barcoding.

2. 1. Methods to Prepare DNA for Efficient Massive Sequencing

   Author : Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
   Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; TEKNIK OCH TEKNOLOGIER; MEDICIN OCH HÄLSOVETENSKAP; ENGINEERING AND TECHNOLOGY; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

   Abstract : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. READ MORE

4. 2. Technologies for Single Cell Genome Analysis

   Author : Erik Borgström; Afshin Ahmadian; Sten Linnarsson; KTH; []
   Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

   Abstract : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. READ MORE

5. 3. Barcoded DNA Sequencing for Parallel Protein Detection

   Author : Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
   Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

   Abstract : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. READ MORE

6. 4. Phasing single DNA molecules with barcode linked sequencing

   Author : David Redin; Afshin Ahmadian; Ulf Gyllensten; KTH; []
   Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single molecule sequencing; DNA barcoding; whole genome haplotyping; linked-read sequencing; phasing; de novo genome assembly.; Biotechnology; Bioteknologi;

   Abstract : Elucidation of our genetic constituents has in the past decade predominately taken the form of short-read DNA sequencing. Revolutionary technology developments have enabled vast amounts of biological information to be obtained, but from a medical standpoint it has yet to live up to the promise of associating individual genotypes to phenotypic states of wide-spread clinical relevance. READ MORE

7. 5. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes

   Author : Di Wu; Masood Kamali-Moghaddam; Ulf Landegren; Ola Söderberg; Jörg Hoheisel; Uppsala universitet; []
   Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

   Abstract : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. READ MORE