Search for dissertations about: "DNA binding protein"

Showing result 16 - 20 of 543 swedish dissertations containing the words DNA binding protein.

  2. 16. Structural Adaptation of DNA to Small Ligands and Recombination Proteins

    Author : Hye-Kyung Kim; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; triplex DNA; groove binders; intercalators; SANS; DNA; optical spectroscopy; recombination proteins;

    Abstract : In order to contribute to the understanding of mechanisms of recognition in nucleic acid systems, the interaction of DNA with a variety of ligands, representing different cases of adaptation of the double-helix structure, was studied using flow linear dichroism (LD), circular dichroism (CD), fluorescence and absorption spectroscopic techniques. A chiral hydrophobic metal complex, [Ru(dpphen)3]2+ was examined, since this had been reported to be the most efficient molecule to discriminate DNA handedness. READ MORE

  4. 17. DNA and protein interactions of mouse polyomavirus large T antigen

    Author : Ola Rönn; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; T-antigen; DNA-binding; biosensor; enhancer; Biokemi; Biochemistry; Biokemi; Medical Virology; medicinsk virologi;

    Abstract : Polyomaviruses require dividing cells to multiply. The regulatory genes express proteins, theT-antigens, necessary for this to occur. Large T-antigen (LT), is a phosphoprotein withmultiple intrinsic activities, e.g. READ MORE

  5. 18. Recombinant Enzymes in Pyrosequencing Technology

    Author : Nader Nourizad; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Abstract : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. READ MORE

  6. 19. Towards simulating fully charged protein-DNA systems in water : Zif268-DNA as a test case

    Author : Git Roxström; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Physics; Molecular Dynamics simulation; Zif268; zinc fingers; GROMOS; linkers; recognition sites; Fysik; Physics; Fysik; Physical Chemistry; fysikalisk kemi;

    Abstract : In this thesis, we develop a computing strategy to simulate fully charged protein-DNA systems with a general force field (GROMOS87). The ions neutralizing the phosphates (P-ions) are submitted to global symmetry constraints. A model exhibiting ionic-atmosphere effects on mobile P-ions was implemented via very weak restraint forces. READ MORE

  7. 20. Unravelling Mechanistic Insights of Eukaryotic Transcriptional Control

    Author : Johanna Hörberg; Göteborgs universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Transcriptional control; Gene expression regulation; DNA supercoiling; DNA methylation; DNA-protein recognition; Basic-Leucine-Zipper transcription factors; Basic-Helix-Loop-Helix transcription factors; Sequence specific DNA plasticity; Transcription factor cooperativity; Cancer; Molecular modelling; Molecular dynamics;

    Abstract : DNA transcription involves the association of multiple proteins with DNA to convert the genetic information into messenger-RNA transcripts, which later will be translated into proteins. Con-trolled regulation of transcription is imperative for preserving the healthy and homeostatic state of cells. READ MORE