Search for dissertations about: "DNA load"

Showing result 1 - 5 of 80 swedish dissertations containing the words DNA load.

  2. 1. DNA vaccines and bacterial DNA in immunity

    Author : Lisa Charlotta Bandholtz; Karolinska Institutet; Karolinska Institutet; []
    Keywords : DNA vaccine; CpG DNA; antimicrobial peptides C. pneumoniae; Shigella spp;

    Abstract : This thesis describes DNA-based vaccination and the importance of bacterial DNA in different immunological perspectives. Intranasal (i.n.) DNA vaccination utilizing a plasmid encoding the chlamydial heat shock protein 60 (p-hsp-60) generated lower bacterial burden and reduced pathology in the lungs of mice after subsequent infection with C. READ MORE

  4. 2. Functional and structural properties of eukaryotic DNA polymerase epsilon

    Author : Olga Chilkova; Erik Johansson; Per Elias; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; eukaryotic DNA replication; DNA polymerase epsilon; protein-DNA interaction; Biochemistry; Biokemi;

    Abstract : In eukaryotes there are three DNA polymerases which are essential for the replication of chromosomal DNA: DNA polymerase alpha (Pol alpha), DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon). In vitro studies of viral DNA replication showed that Pol alpha and Pol delta are sufficient for DNA replication on both leading and lagging DNA strands, thus leaving the function of Pol epsilon unknown. READ MORE

  5. 3. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    Author : Ingrid Ziegler; Kristoffer Strålin; Paula Mölling; Magnus Rasmussen; Örebro universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Abstract : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. READ MORE

  6. 4. Methods for deep examination of DNA

    Author : Mårten Neiman; Karolinska Institutet; Karolinska Institutet; []
    Keywords : massive parallel sequencing;

    Abstract : The development of sequencing technology has had a rapid pace during the last years and today, the sequencing instruments harbors enormous capacity. This thesis is about the development of methods to make the most out of this capacity and to use it for various applications. READ MORE

  7. 5. HIV therapies : from health-related quality of life to DNA levels

    Author : Lars E Eriksson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : AIDS; antiretroviral therapy; CD4; DNA load; HIV; immunotherapy; viral kinetics; nursing; medical care; quality of life; real-time PCR; rgpl60; RNA load; therapy interruption.;

    Abstract : The treatment of people infected with HIV was revolutionised with the introduction of protease inhibitor based antiretroviral combination therapy. The therapy has greatly reduced the incidence of AIDS and AIDS-related mortality in the industrialised world. READ MORE