Search for dissertations about: "DNA shuffling"

Showing result 6 - 10 of 10 swedish dissertations containing the words DNA shuffling.

  2. 6. The Quest for Functional Quasi-Species in Glutathione Transferase Libraries

    Author : Arna Rúnarsdóttir; Bengt Mannervik; Ralf Morgenstern; Uppsala universitet; []
    Keywords : glutathione transferase; directed evolution; multivariate analysis; quasi-species; isothiocyanates; Biokemi; Biochemistry;

    Abstract : Glutathione transferases (GSTs) are good candidates for investigations of enzyme evolution, due to their broad substrate specificities and structural homology. The primary role of GSTs is to act as phase II detoxifying enzymes protecting the cell from toxic compounds of both endo- and exogenous origins. READ MORE

  4. 7. Directed Enzyme Evolution of Theta Class Glutathione Transferase : Studies of Recombinant Libraries and Enhancement of Activity toward the Anticancer Drug 1,3-bis(2-Chloroethyl)-1-nitrosourea

    Author : Anna-Karin Larsson; Bengt Mannervik; Ian Cotgreave; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; directed evolution; 1; 3-bis 2-chloroethyl -1-nitrosourea; alkyltransferase; recombinant libraries; chloroethylnitrosourea; multi-dimensional data analysis; Biokemi; Biochemistry; Biokemi;

    Abstract : Glutathione transferases (GSTs) are detoxication enzymes involved in the cellular protection against a wide range of reactive substances. The role of GSTs is to catalyze the conjugation of glutathione with electrophilic compounds, which generally results in less toxic products. READ MORE

  5. 8. Gene conversion of the mouse MHC class II

    Author : Kari Högstrand; Ulf Gyllensten; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MHC; H2; gene conversion; evolution; polymorphism; Immunology; Immunologi; immunologi; Immunology;

    Abstract : The major histocompatibility complex (MHC) is the most polymorphic vertebrate gene loci known to exist. Gene products of the MHC are responsible for presentation of antigen derived peptides to T cells of the immune system, which thereby become activated and involved in the elimination of particles recognised as non-self. READ MORE

  6. 9. Novel allergen preparations for use in allergen-specific immunotherapy

    Author : Theresa Neimert Andersson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Allergy; allergen-specific immunotherapy; adjuvants; carbohydrate-based particles; allergic asthma; mouse models; hypoallergens;

    Abstract : Allergy is a common disease in the industrialized countries, affecting approximately 25% of the population. Therefore, there is a need to find new treatment strategies to improve the quality of life for allergic individuals. Today the only treatment that gives long-lasting reduction of allergic symptoms is allergen-specific immunotherapy (SIT). READ MORE

  7. 10. Autoantigenic properties of the U1-70K protein

    Author : Elisabet Welin Henriksson; Karolinska Institutet; Karolinska Institutet; []
    Keywords : U1-70K protein; ribonucleoproteins; autoantigens; autoantibodies; epitopemapping; connective tissue disease; species specificity; cross reactions; amino acidsequence; recombinant proteins; mutagenesis;

    Abstract : Patients with the autoimmune diseases systemic lupus erythematosus and mixed connective tissue disease produce autoantibodies directed towards specific groups of cellular constituents in their own body. Two of the targeted antigens are denoted Sm and RNP, and consist of distinct collections of splicing associated RNA-protein complexes. READ MORE