Search for dissertations about: "DNA transfection"

Showing result 1 - 5 of 66 swedish dissertations containing the words DNA transfection.

  2. 1. Controlling DNA compaction and the interaction with model biomembranes

    Author : Marie-Louise Ainalem; Fysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; model biomembrane; DNA compaction; cryo-TEM; null ellipsometry; dynamic light scattering; toroid; bilayer; neutron reflectivity; dendrimer; PAMAM; DNA; poly amido amine ;

    Abstract : The studies described in this thesis forms part of a larger collaborative project, Neonuclei, with the objective to design a module for packaging the negatively charged DNA into a ”neonucleus” with transcription competence. There are many applications that require control of DNA compaction, i.e. packaging or condensation. READ MORE

  4. 2. Condensation of DNA for Gene Delivery: Studies on Vector-Nucleic Acid Interactions and Transfection Efficiency

    Author : Kristina Fant; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; dendrimer; endocytosis; uptake; gene delivery; optical spectroscopy; structure-activity relationship; DNA; cell-penetrating peptide;

    Abstract : This thesis concerns the delivery of nucleic acids into cells and tissues with the aim to change or correct gene expression. The field of gene delivery has the ultimate goal of producing genetic drugs for clinical applications, and the activity in this area is intense although so far the use is limited to research purposes. READ MORE

  5. 3. Functional Cyclic Carbonate Monomers and Polycarbonates : Synthesis and Biomaterials Applications

    Author : Jonas Mindemark; Tim Bowden; Andrew Dove; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA condensation; gene delivery; ionomers; polyplexes; self-assembly; amphiphiles; biodegradable; biological applications of polymers; transfection; cyclic carbonate monomers; polycarbonates; semicrystalline polymers; click chemistry; triazoles; cycloaddition; Kemi med inriktning mot polymerkemi; Chemistry with specialization in Polymer Chemistry;

    Abstract : The present work describes a selection of strategies for the synthesis of functional aliphatic polycarbonates. Using an end-group functionalization strategy, a series of DNA-binding cationic poly(trimethylene carbonate)s was synthesized for application as vectors for non-viral gene delivery. READ MORE

  6. 4. Comparative Cell Biology in Diplomonads

    Author : Elin Einarsson; Staffan Svärd; Scott Dawson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Giardia intestinalis; Spironucleus salmonicida; intestinal parasite; hydrogenosome; encystation; gene regulation; transfection; diplomonad; antigenic variation; annexin;

    Abstract : The diplomonads are a diverse group of eukaryotic flagellates found in microaerophilic and anaerobic environments. The most studied diplomonad is the intestinal parasite Giardia intestinalis, which infects a variety of mammals and cause diarrheal disease. READ MORE

  7. 5. On the Regulation of Ornithine Decarboxylase

    Author : Eva Lövkvist Wallström; Institutionen för experimentell medicinsk vetenskap; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Fysiologi; Physiology; Crithidia fasciculata.; polyamines; Ornithine decarboxylase; translational control; osmolarity; untranslated region; COS cells; CHO cells; transfection; protein turnover; PEST region;

    Abstract : The polyamines putrescine, spermidine and spermine have been shown to be involved in cell growth and differentiation, and are considered to be crucial components of the living cell. The synthesis of polyamines in the cell is strictly regulated. READ MORE