Search for dissertations about: "Desorption Electrospray Ionization"

Showing result 6 - 10 of 27 swedish dissertations containing the words Desorption Electrospray Ionization.

  2. 6. Sample preconcentration in open microchannels : Combinations with MALDI and nano-ESI mass spectrometry and computer simulations

    Author : Saara Mikkonen; Åsa Emmer; Leopold Ilag; Jenny Ottosson Takanen; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Preconcentration; open microchannel; microchip; MALDI; electrospray ionization; nanoelectrospray; mass spectrometry; computer simulation;

    Abstract : In this thesis a novel concept for preconcentration of biomolecules in open microchannels is presented. The preconcentration is based on electromigration of charged analytes, and detection is performed with matrix-assisted laser desorption/ionization (MALDI) or nano-electrospray ionization (nESI) mass spectrometry (MS). READ MORE

  4. 7. Method and technique development in peptide and protein mass Spectrometry

    Author : Kristina Håkansson; Uppsala universitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; electrospray ionization; peptide sequencing; Fourier transform ion cyclotron resonance; hydrogen deuterium exchange; protein structure; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Mechanistic studies and applicability of several methods and techniques for studying peptides and proteins with mass spectrometry are presented. A model for the protein molecular ion formation in explosive matrix-assisted plasma desorption mass spectrometry is proposed, involving a contribution from the chemical energy release during conversion of the explosive, an enhanced protonation through collisions with products from the explosive decay, and electron scavenging by other products. READ MORE

  5. 8. Tools for applied soft ionization mass spectrometry

    Author : Joakim Romson; Åsa Emmer; Peter Dinér; Steven Wilson; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; Electrospray ionization; Matrix assisted laser desorption ionization; Mass accuracy; Mass shifts; Ion trap; Capillary electrophoresis; Sample concentration; Masspektrometri; Elektrospray-jonisering; Matrisassisterad laser desorbtion jonisering; Mass-exakthet; Masskift; Jonfälla; Kapillärelektrofores; Provkoncentration; Kemi; Chemistry;

    Abstract :  Mass spectrometry (MS) is an important analytical tool. Its most importantadvantages are sensitivity, resolution, and accurate and precise m/zmeasurements. To get the best results from an instrument, the user musthave knowledge of its limitation and requirements. READ MORE

  6. 9. Mass spectrometry of biopolymers based on ion-induced desorption

    Author : Roman Alexandrovich Zubarev; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Radiation sciences; Strålningsvetenskap; Radiation biology; Strålningsbiologi; Ion Physics; jonfysik;

    Abstract : Various aspects of biopolymer molecular characterization by their molecular masses havebeen studied, with emphasis on ion-induced desorption and time-of-flight mass Spectrometry(TOF MS). Limitations of the average-isotopic mass concept, as well as the utility ofaccurate monoisotopic mass measurements for deriving amino-acid and elementalcompositions, are discussed. READ MORE

  7. 10. Development and application of alternative methods for profiling proteins N-glycosylation

    Author : Alessandro Quaranta; Leopold Ilag; Niclas Karlsson; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; N-glycosylation; Glycomics; Glycosylation Biomarkers; Intact Glycoproteins; Glycoform Quantification; Mass Spectrometry; Ion Mobility Spectrometry; MALDI-MS; Paper Spray Ionization; Microfluidics; Magnetic Beads; Immunoaffinity Purification; Nanobodies; Analytical Chemistry; analytisk kemi;

    Abstract : Glycosylation is a post-translational modification (PTM) that exerts profound structural and functional effects on the modified protein. Glycan synthesis and conjugation to proteins are regulated by a myriad of factors, both genetic and environmental, and are also influenced by external stressors. READ MORE