Search for dissertations about: "Detergent"

Showing result 1 - 5 of 115 swedish dissertations containing the word Detergent.

  1. 1. Selective extraction systems for detection and purification of membrane proteins

    Author : Ulf Sivars; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Abstract : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. READ MORE

  2. 2. NMR studies on interactions between the amyloid β peptide and selected molecules

    Author : Anna Wahlström; Astrid Gräslund; Göran Karlsson; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Amyloid β peptide; Alzheimer s disease; Aggregation; Oligomer; Amyloid; Interaction; Nuclear Magnetic Resonance Spectroscopy; Circular Dichroism Spectroscopy; Thioflavin T; Detergent; Congo red; Affibody; Cyclodextrin dimer; Metal ion; Chemistry; Kemi; Biochemistry; Biokemi; Biophysics; biofysik;

    Abstract : Alzheimer’s disease is an incurable neurodegenerative disorder linked to the amyloid β (Aβ) peptide, a 38-43 residue peptide. The detailed molecular disease mechanism(s) is (are) unknown, but oligomeric Aβ structures are proposed to be involved. READ MORE

  3. 3. Intracellular vesicles induced by monotopic membrane protein in Escherichia coli

    Author : Hanna M. Eriksson; Åke Wieslander; Meta Kuehn; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; intracellular vesicles; Escherichia coli; glycosyltransferase; overexpression; optimization; detergent; screening; lipid composition; Biochemistry; Biokemi; biokemi; Biochemistry;

    Abstract : The monotopic membrane protein alMGS, a glycosyltransferase catalyzing glucolipid synthesis in Acholeplasma laidlawii, was overexpressed in Escherichia coli. Optimization of basic growth parameters was performed, and a novel method for detergent and buffer screening using a small size-exclusion chromatography was developed. READ MORE

  4. 4. Partitioning of Drugs and Lignin Precursor Models into Artificial Membranes

    Author : Elisabet Boija; Gunnar Johansson; Per Lundahl; Christine Lagerquist Hägglund; Marja-Liisa Riekkola; Uppsala universitet; []
    Keywords : Biochemistry; Bilayer disk; Capillary electrophoresis; Detergent; Drug; Electrostatic interaction; Hydrophobic interaction; Immobilized liposome chromatography; Lignin; Lignin precursor model; Liposome; Membrane model; Octanol water partitioning; Partitioning; Phospholipid; Phospholipid bilayer; Sterol; Biokemi;

    Abstract : The main aim of this thesis was to characterize membrane-solute interactions using artificial membranes in immobilized liposome chromatography or capillary electrophoresis. The partitioning of a solute into a cell membrane is an essential step in diffusion across the membrane. READ MORE

  5. 5. Taming the Griffin : Membrane interactions of peripheral and monotopic glycosyltransferases and dynamics of bacterial and plant lipids in bicelles

    Author : Jobst Liebau; Lena Mäler; Charles Sanders; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; membrane; bicelle; lipid; detergent; lipopolysaccharide; glycosyltransferase; WaaG; fluorescence; circular dichroism; NMR; paramagnetic relaxation enhancement; model-free approach; dynamics; Biophysics; biofysik;

    Abstract : Biological membranes form a protective barrier around cells and cellular compartments. A broad range of biochemical processes occur in or at membranes demonstrating that they are not only of structural but also of functional importance. One important class of membrane proteins are membrane-associated glycosyltransferases. READ MORE