Search for dissertations about: "Diagnostic PCR"

Showing result 1 - 5 of 128 swedish dissertations containing the words Diagnostic PCR.

  1. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    University dissertation from Applied Microbiology (LTH)

    Author : Rickard Knutsson; [2001]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE

  2. 2. Tularemia epidemiological, clinical and diagnostic aspects

    University dissertation from Örebro : Örebro universitet

    Author : Henrik Eliasson; Erik Bäck; Anders Sjöstedt; Clas Ahlm; [2008]
    Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Francisella; tularemia; epidemiology; case-control; diagnosis; PCR; clinical characteristics; immunology; ELISA; lymphocyte stimulation; VETERINARY MEDICINE Medicine; VETERINÄRMEDICIN Medicin; Infektionssjukdomar; Infectious Diseases;

    Abstract : Tularemia is a zoonosis caused by the small, fastidious, gram-negative rod Francisella tularensis that appears over almost the entire Northern Hemisphere. In Sweden, tularemia has appeared mainly in restricted areas in northern parts of central Sweden. READ MORE

  3. 3. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Amal Elfaitouri; Uppsala universitet.; [2006]
    Keywords : Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Abstract : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. READ MORE

  4. 4. Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

    University dissertation from Department of Applied Microbiology, Lund University

    Author : Waleed Abu Al-Soud; [2000]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; virology; bacteriology; Microbiology; AmpliTaq Gold; Amplification facilitators; Betaine; Blood culture medium; Blood; BSA; cheese; faeces; gp32; Haemoglobin; Ions; Immunoglobulin G; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. READ MORE

  5. 5. Molecular biological techniques as a tool in diagnostic pathology : Applications in B-cell lymphoproliferative disease, medullary thyroid carcinoma and cervical carcinoma

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Mansour Alemi; [2000]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; PCR-SSCP; MTC; RET mutation; Lymphoma; HPV; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Pathology; patologi;

    Abstract : Identification of malignancy associated with mutations in gene sequences requires detection ofas little as a single base difference. A powerful technique in mutation detection is polymerasechain reaction (PCR) followed by single-strand conformational polymorphism (SSCP) andsequencing. READ MORE