Search for dissertations about: "Diagnostic PCR"

Showing result 1 - 5 of 125 swedish dissertations containing the words Diagnostic PCR.

  1. 1. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    University dissertation from Applied Microbiology (LTH)

    Author : Rickard Knutsson; Lund University.; Lunds universitet.; [2001]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. READ MORE

  2. 2. Tularemia epidemiological, clinical and diagnostic aspects

    University dissertation from Örebro : Örebro universitet

    Author : Henrik Eliasson; Örebro universitet.; [2008]
    Keywords : LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Francisella; tularemia; epidemiology; case-control; diagnosis; PCR; clinical characteristics; immunology; ELISA; lymphocyte stimulation; VETERINARY MEDICINE Medicine; VETERINÄRMEDICIN Medicin; Infektionssjukdomar; Infectious Diseases;

    Abstract : Tularemia is a zoonosis caused by the small, fastidious, gram-negative rod Francisella tularensis that appears over almost the entire Northern Hemisphere. In Sweden, tularemia has appeared mainly in restricted areas in northern parts of central Sweden. READ MORE

  3. 3. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Amal Elfaitouri; Uppsala universitet.; [2006]
    Keywords : Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Abstract : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. READ MORE

  4. 4. Helicobacter pylori-induced gastritis in guinea pigs: Model development, diagnostic methods and comparison with mouse protocols

    University dissertation from Department of Laboratory Medicine, Lund University

    Author : Håkan Sjunnesson; Lund University.; Lunds universitet.; [2002]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; bakteriologi; Mikrobiologi; virologi; mykologi; Microbiology; bacteriology; virology; mycology;

    Abstract : Helicobacter pylori is a gram-negative spiral-shaped bacterium that colonizes the human stomach and causes gastric inflammation, gastric and duodenal peptic ulcers, gastric cancer and MALT-lymphoma. A novel guinea pig model of H. pylori infection was developed. READ MORE

  5. 5. Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

    University dissertation from Department of Applied Microbiology, Lund University

    Author : Waleed Abu Al-Soud; Lund University.; Lunds universitet.; [2000]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; virology; bacteriology; Microbiology; AmpliTaq Gold; Amplification facilitators; Betaine; Blood culture medium; Blood; BSA; cheese; faeces; gp32; Haemoglobin; Ions; Immunoglobulin G; Mikrobiologi; bakteriologi; virologi; mykologi;

    Abstract : PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. READ MORE