Search for dissertations about: "Dna extraction"

Showing result 16 - 20 of 73 swedish dissertations containing the words Dna extraction.

  2. 16. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Author : Cecilia Kepka; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Abstract : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. READ MORE

  4. 17. 32P-postlabelling analysis of complex DNA adducts in human tissues

    Author : Ke Yang; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Complex DNA adducts in humans originate from exogenous and endogenous sources. Polycyclic aromatic hydrocarbons (PAHs) are a large group of chemicals including many potent carcinogens. PAHs are ubiquitously present in the environment and PAH-DNA adducts have been extensively studied in humans. READ MORE

  5. 18. Palaeogenomic reconstruction of woolly mammoth evolutionary history and extinction dynamics

    Author : Marianne Dehasque; Love Dalén; David Díez-del-Molino; Rike Stelkens; Rebecca N. Johnson; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; woolly mammoth; Mammuthus primigenius; extinction; Wrangel Island; ancient DNA; palaeogenomics; radiocarbon dating; structural variants; genome erosion; population structure; Systematic Zoology; zoologisk systematik och evolutionsforskning;

    Abstract : Biodiversity is declining globally. Yet, the biological and genetic processes associated with these declines on a longer timescale are still poorly understood. Ancient DNA is a powerful tool to study evolution in real-time. Despite advances in the field, there is further need for refinement of laboratory and computational techniques. READ MORE

  6. 19. QCM-D with focus on variations in oscillation amplitude

    Author : Malin Edvardsson; Chalmers tekniska högskola; []
    Keywords : shear oscillation; actuator; Quartz crystal microbalance; amplitude; streptavidin; lipid vesicles; biosensor; colloidal particles; size distribution; QCM-D; protein binding; DNA hybridization; dual harmonic; lipid bilayer formation;

    Abstract : The QCM-D (quartz crystal microbalance with dissipation monitoring) technique is an increasingly popular tool in studies of molecular interactions at interfaces, e.g. binding/unbinding reactions at surfaces, studies of thin film properties, and conformational changes. READ MORE

  7. 20. From Tissue to Mutations : Genetic Profiling of Colorectal Cancer

    Author : Lucy Mathot; Tobias Sjöblom; Mats Nilsson; Alberto Bardelli; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Nucleic acid extraction; genotyping; targeted sequencing; mutation analysis; colorectal cancer; metastatic disease; Medical Science; Medicinsk vetenskap;

    Abstract : Comprehensive characterisation of the mutational landscapes of solid tumours is a multistep process involving the collection of suitable samples, the extraction of nucleic acids and the preparation of these materials for mutational analyses. In this thesis, I aimed to develop a streamlined process for the analysis of colorectal cancer (CRC) patient samples in order to identify novel mutations that hallmark the development of advanced disease. READ MORE