Search for dissertations about: "Droplet PCR"

Showing result 1 - 5 of 11 swedish dissertations containing the words Droplet PCR.

  1. 1. Droplet Microfluidics reverse transcription and PCR towards Single Cell and Exosome Analysis

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Lovisa Söderberg; KTH.; [2017]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Droplet microfluidics; Reverse transcription; Droplet PCR; High Throughput biology; Single cell Analysis; Exosomes; Biotechnology; Bioteknologi;

    Abstract : Miniaturization of biological analysis is a trend in the field of biotechnology aiming to increase resolution and sensitivity in biological assays. Decreasing the reaction volumes to analyze fewer analytes in each reaction vessel enables the detection of rare analytes in a vast background of more common variants. READ MORE

  2. 2. Droplet microfluidics for single cell and nucleic acid analysis

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Prem Kumar Periyannan Rajeswari; KTH.; [2016]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Biotechnology; Bioteknologi;

    Abstract : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. READ MORE

  3. 3. Quantitative detection of bacterial DNA in whole blood in bloodstream infection

    University dissertation from Örebro : Örebro University

    Author : Ingrid Ziegler; Örebro universitet.; [2018]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bloodstream infection; bacteremia; sepsis; DNA load; quantitative PCR; droplet digital PCR; 16S rDNA;

    Abstract : This thesis aims to increase the knowledge on how quantitative PCR can be used in the diagnostics of bloodstream infections, with an emphasis on quantitative elements.In Papers I and II, we evaluated quantitative data from two commercial PCR tests for pathogen detection directly in blood, Magicplex Sepsis (I) and SeptiFast (II), from patients with suspected sepsis. READ MORE

  4. 4. Improved Techniques for High-Throughput Molecular Diagnostics

    University dissertation from Stockholm : Kemi

    Author : Mario Curcio; KTH.; [2002]
    Keywords : DNA; Molecular Diagnostics;

    Abstract : The amount of information derived from sequencing the humangenome is leading to an exponential increase in the rate atwhich genes and genetic disorders are mapped and characterized.As a consequence, the demand for genetic testing is alsodramatically increasing. READ MORE

  5. 5. Molecular Analysis of Breast Cancer Transcriptomes, Genomes, and Circulating Tumor DNA

    University dissertation from Division of Oncology and Pathology

    Author : Eleonor Olsson; Lunds universitet.; Lund University.; Lunds universitet.; Lund University.; [2015]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cancer stem cells; RNA splicing; breast cancer; mutations; circulating tumor DNA; early detection of recurrent breast cancer; sequencing;

    Abstract : Breast cancer is a very heterogeneous disease in terms of clinical characteristics, genetic aberrations and prognosis. In Paper I, we focused on the CD44 molecule that often is aberrantly expressed in breast cancer and is widely used as a marker for cancer stem cells. READ MORE