Search for dissertations about: "EF-hand"

Showing result 1 - 5 of 12 swedish dissertations containing the word EF-hand.

  1. 1. Exploring the interactome of EF-hand proteins

    Author : Mikael Bauer; [2010]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; EF-hand; calcium; STIM; stromal interaction molecule; secretagogin; Calmodulin; calbindin D28k; protein-protein interactions; zinc; protein array;

    Abstract : EF-hand proteins are central in many cellular processes being the main group of Ca2+-sensing and buffering proteins in the cell. In the work presented in this thesis we study ligand interactions of three EF-hand proteins; calmodulin, calbindin D28k and secretagogin. READ MORE

  2. 2. Shaking the EF-Hand. Dynamics and Ion-Binding to Calmodulin and Calbindin D9k

    University dissertation from Anders Malmendal, Physical Chemistry 2, Lund University, P.O.Box 124, S-221 00 Lund, Sweden

    Author : Anders Malmendal; [1998]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; magnesium; line shape; ion binding; EF-hand; cooperativity; conformational exchange; chemical shift; calmodulin; calcium; calbindin D9k; allostery; backbone dynamics; NMR; relaxation; Physical chemistry; Fysikalisk kemi;

    Abstract : The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. The x-ray structure of magnesium-saturated calbindin D9k shows a magnesium ion in only one of the two ion binding sites. READ MORE

  3. 3. The effect of sequence truncation and amino-acid substitutions on the biophysical properties of protein S and calbindin D9k

    University dissertation from Karin Julenius, Physical Chemistry 2, Lund University, P.O.B. 124, S-221 00 Lund, Sweden

    Author : Karin Julenius; [1999]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; surface plasmon resonance; fluorescence spectroscopy; circular dichroism spectroscopy; NMR spectroscopy; hydrophobic effect; protein stability; dimerization; cooperativity; calcium binding; EGF-module; EF-hand; calbindin D9k; protein S; synthet; Physical chemistry; Fysikalisk kemi;

    Abstract : Protein S is a modular protein and a cofactor in the protein C anticoagulant system. It consists of a Gla-module, a thrombin-sensitive loop, four EGF-modules and a SHBG-like module. READ MORE

  4. 4. INOSITOL PHOSPHOLIPID-BASED SIGNAL TRANSDUCTION IN PLANTS Phosphoinositide-Specific Phospholipase C and 3-Phosphoinositide Dependent Protein Kinase-1 in Arabidopsis thaliana

    University dissertation from KF Sigma AB, Lund

    Author : Lotta Otterhag; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Lipids; steroids; membranes; Lipider; steroider; mebran; phospholipase C; enzymologi; Proteiner; enzymology; Proteins; Växtbiokemi; Plant biochemistry; phosphorylation; PDK1; calcium; EF-hand; plasma membrane; Arabidopsis thaliana;

    Abstract : Phosphoinositides (PIs) play major roles in cell signal transduction. Although it is clear that PIs are important components also in plant signalling, little is known about their roles in plant cells, compared to animal cells. READ MORE

  5. 5. Protein Dynamics Studied by NMR Spin Relaxation. Conformational Transitions of a Calmodulin Mutant

    University dissertation from Department of Biophysical Chemistry, Lund University

    Author : Patrik Lundström; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molekylär biofysik; Molecular biophysics; spin relaxation; rotating-frame; NMR; multiple-quantum; EF-hand; chemical shift; chemical exchange; calcium binding proteins; calmodulin;

    Abstract : Binding of calcium to the protein calmodulin leads to molecular reorganization that enables interaction with target peptides and activation of downstream processes. I have studied the dynamics of the calcium-loaded form of a C-terminal calmodulin mutant (E140Q-Tr2C) using NMR spin relaxation experiments. READ MORE