Search for dissertations about: "EIAV"

Showing result 1 - 5 of 6 swedish dissertations containing the word EIAV.

  2. 1. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Author : Johan Nord; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Abstract : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. READ MORE

  4. 2. Posttranscriptional regulation of gene expression in retroviruses and human papillomaviruses

    Author : Wei Tan; Karolinska Institutet; Karolinska Institutet; []
    Keywords : CTE; EIAV; HIV- 1; HPV; Rev; RRE; splice site; SRV- 1; 3 UTR Stockholm 1996 Doctoral Thesis;

    Abstract : Human immunodeficiency virus type I (HIV-1) is the prototype of complex retroviruses, whereas human papillomaviruses (HPVs) are nonenveloped, cricular double-stranded DNA viruses. The HIV-1 Rev protein is a posttranscriptional regulator, which acts on its target RNA sequence termed the Rev-responsive element (RRE) to facilitate nuclear export of unspliced and partially spliced HIV-1 mRNAs, and to improve their stability and translatability. READ MORE

  5. 3. Regulation of gene and protein expression : two model systems

    Author : Lars H Lund; Karolinska Institutet; Karolinska Institutet; []
    Keywords : HIV-1; EIAV; Tat; TAR; Cyclin T1; CDK9; P-TEFb; RNA polymerase II; gene regulation; transcriptional elongation; CEA; signal sequence.;

    Abstract : Genetic information is expressed by DNA transcription and mRNA translation. With few exceptions, every cell of an organism, or the human body, contains the same genetic information. Yet the structure and function of different tissues are highly variable. READ MORE

  6. 4. Viral dUTPases, Recombinant Expression, Purification and Characterization

    Author : Anna-Carin Bergman; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Proteins; nucleocapsid protein; retrovirus; herpesvirus; dUTPase; nucleotide metabolism; enzymology; Proteiner; enzymologi;

    Abstract : The enzyme deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. READ MORE

  7. 5. Homotrimeric dUTPases : Principles of Catalysis and Inhibitor Design

    Author : Lorena Gonzalez Palmén; Jan Kvassman; Dolores Gonzalez-Pacanowska; Högskolan i Kalmar; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; dUTPases; kinetics; ground state destabilization; inhibitors; Biochemistry; Biokemi; Natural Science; Naturvetenskap;

    Abstract : The ubiquitous enzyme dUTPase hydrolyzes dUTP into dUMP and pyrophosphate, preventing DNA fragmentation and cell death due to accumulation of dUTP. Inhibitors of dUTPase could serve as drugs in the treatment of cancers and infectious diseases. This thesis presents five studies. READ MORE