Search for dissertations about: "ELISA immunoassay"

Showing result 1 - 5 of 24 swedish dissertations containing the words ELISA immunoassay.

  1. 1. Rapid sample preparation and bioanalytical techniques for efficient screening of organic pollutants in the environment

    Author : Malin Nording; Peter Haglund; Ron Hoogenboom; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; accelerated solvent extraction; CAFLUX; food; immunoassay; PAH; PCDD; PCDF; pressurized liquid extraction; soil; CALUX; cell-based bioassay; chemically activated fluorescent gene expression; chemically activated luciferase gene expression; dioxin; ELISA; enzyme-linked immunosorbent assay; feed; Environmental chemistry; Miljökemi;

    Abstract : Large numbers of samples often need to be prepared and analysed in surveys of organic pollutants in the environment, but while the methods commonly used in such surveys can provide abundant detail they are generally costly, time-consuming and require large amounts of resources, so there is a need for simpler techniques. The work underlying this thesis assessed the potential utility of more convenient sample preparation and bioanalytical techniques for rapidly screening various environmental matrices that could be useful complements to higher resolution methods. READ MORE

  2. 2. Antibodies and Antibody Mimics in Binding Assays

    Author : Ioana Wärnmark; Tillämpad biokemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Polymerteknik; biopolymers; ELISA; antibody; molecularly imprinted polymer; Biochemistry; Biokemi; Metabolism; metabolism; Biokemisk teknik; Polymer technology; Biochemical technology;

    Abstract : This thesis presents development of new analytical methods using antibodies and molecularly imprinted polymers (MIPs) in binding assays: 1) The high specificity of antibodies and the wide variety of efficient labels make immunoassay one of the most popular analytical techniques today. Enzyme-linked immunosorbent assay (ELISA) is most widely used, since the enzyme label can be used as a catalyst to provide very high signal amplification. READ MORE

  3. 3. Real-time PCR and its application for protein quantification

    Author : Kristina Lind; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

    Abstract : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. READ MORE

  4. 4. Human antibody responses to hantavirus recombinant proteins & development of diagnostic methods

    Author : Fredrik Elgh; Umeå universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Puumala virus; hantavirus infections; recombinant proteins; capsid; humoral immunity; immunoassay;

    Abstract : Rodent-borne hantaviruses (family Bunyaviridae) cause two distinct human infections; hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). HFRS is a common viral zoonosis, characterized by fever, renal dysfunction and hemostatic imbalance. READ MORE

  5. 5. Biosensors and Automation for Bioprocess Monitoring and Control

    Author : M A Kumar; Bioteknik; []
    Keywords : online monitoring; ELISA; immunosensor; flow injection; Biosensor; bioprocess; control; environmental;

    Abstract : Bioprocess monitoring and control is a complex task that needs rapid and reliable methods which are adaptable to continuous analysis. Process monitoring during fermentation is widely applicable in the field of pharmaceutical, food and beverages and wastewater treatment. READ MORE