Search for dissertations about: "EVB"

Showing result 11 - 12 of 12 swedish dissertations containing the word EVB.

2. 11. Towards Understanding of Selectivity & Enantioconvergence of an Epoxide Hydrolase

   Author : Åsa Janfalk Carlsson; Mikael Widersten; Uwe T. Bornscheuer; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Epoxide hydrolase; Epoxide; Enantioselectivity; Regioselectivity; Enantioconvergence; Crystal structures; Biocatalysis; Immobilization; Transient kinetics; CASTing; Directed evolution; Biokemi; Biochemistry;

   Abstract : Epoxide hydrolase I from Solanum tuberosum (StEH1) and isolated variants thereof has been studied for mapping structure-function relationships with the ultimate goal of being able to in silico predict modifications needed for a certain activity or selectivity. To solve this, directed evoultion using CASTing and an ISM approach was applied to improve selectivity towards either of the enantiomeric product diols from (2,3-epoxypropyl)benzene (1). READ MORE

4. 12. Computational Protein Evolution : Modeling the Selectivity and Promiscuity of Engineered Enzymes

   Author : Klaudia Szeler; Shina C. Lynn Kamerlin; Mikael Widersten; Maria Ramos; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; enzymes; kinetics; catalysis; density functional theory; empirical valence bond; diesters; evolution; promiscuity; Biokemi; Biochemistry;

   Abstract : Enzymes are biological catalysts that significantly increase the rate of all biochemical reactions that take place within cells and are essential to maintain life. Many questions regarding their function remain unknown. READ MORE