Search for dissertations about: "Electron capture dissociation ECD"

Showing result 1 - 5 of 7 swedish dissertations containing the words Electron capture dissociation ECD.

2. 1. Characterization of Polypeptides by Tandem Mass Spectrometry Using Complementary Fragmentation Techniques

   Author : Michael Lund Nielsen; Roman A. Zubarev; Per Andrén; Ole Nörregaard Jensen; Uppsala universitet; []
   Keywords : Analytical chemistry; Mass Spectrometry; Electron capture dissociation ECD ; Collision-activated dissociation CAD ; Proteomics; Post-translational modifications; De Novo sequencing; Bioinformatics; Analytisk kemi;

   Abstract : In the growing field of proteomics identification of proteins by tandem mass spectrometry (MS/MS) is performed by matching experimental mass spectra against calculated spectra of all possible peptides in a protein database. One problem with this approach is the false-positive identifications. READ MORE

4. 2. Secondary and Higher Order Structural Characterization of Peptides and Proteins by Mass Spectrometry

   Author : Christopher Adams; Roman Zubarev; Göran Possnert; Carlito Lebrilla; Uppsala universitet; []
   Keywords : Engineering physics; Protein Structure; Mass Spectrometry; Electron Capture Dissociation; Tandem Mass Spectrometry; Teknisk fysik;

   Abstract : The work in this thesis has demonstrated the advantages and limitations of using MS based technologies in protein and peptide structural studies. Tandem MS, specifically electron capture dissociation (ECD) have shown the ability to provide structural insights in molecules containing the slightest of all modifications (D-AA substitution). READ MORE

5. 3. Analysis of Complex Biological Samples using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Author : Margareta Ramström; Jonas Bergquist; Per Håkansson; Karin Markides; Carol Robinson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; mass spectrometry; liquid chromatography; protein; proteomics; peptide; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyotrophic lateral sclerosis; electrospray ionization; electron capture dissociation; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Abstract : Studies of protein and peptide expression are vital in order to understand complex biological systems. As demonstrated in this thesis, on-line packed capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) is a useful analytical tool for such studies. READ MORE

6. 4. Strategies to explore the membrane proteome of a cell

   Author : Gianluca Maddalo; Leopold L. Ilag; Peter James; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteomics; Escherichia coli; Enterococcus faecalis; Synechocystis; Mass Spectrometry; BN SDS-PAGE; FT-ICR-MS; Myelin P2 protein; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

   Abstract : The cell envelope plays key roles in numerous processes such as maintaining cellular integrity, communication with other cells, signal transduction, maintenance of cellular homeostasis, and regulation of the traffic of molecules between the cell and the extracellular milieu. Essential membrane components in many of these processes are proteins. READ MORE

7. 5. New Proteomics Methods and Fundamental Aspects of Peptide Fragmentation

   Author : Mikhail Savitski; Roman Zubarev; Per Andren; David Fenyö; Uppsala universitet; []
   Keywords : Bioinformatics; Proteomics; Peptide fragmentation; Bioinformatik;

   Abstract : The combination of collision-activated dissociation, (CAD) and electron capture dissociation, (ECD) yielded a 125% increase in protein identification. The S-score was developed for measuring the information content in MS/MS spectra. This measure made it possible to single out good quality spectra that were not identified by a search engine. READ MORE