Search for dissertations about: "Enrichment PCR"

Showing result 6 - 10 of 25 swedish dissertations containing the words Enrichment PCR.

  2. 6. Microfluidic and Molecular Tools for Genetic Analyses

    Author : Henrik Johansson; Mats Nilsson; Ivo Gut; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microfluidics; PCR; sequencing; cancer genetics; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Medical Genetics; Medicinsk genetik;

    Abstract : Methods that enable interrogation of multiple genomic regions in parallel are very useful for efficient detection of genetic variation. Two different types of probes are described in this thesis that can be used for direct analysis or for sample preparation upstream of Next Generation Sequencing. READ MORE

  4. 7. Tagging systems for sequencing large cohorts

    Author : Mårten Neiman; Afshin Ahmadian; Peter Savolainen; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; next generation sequencing; genotyping; massive parallel sequencing; 454; Pyrosequencing; amplicon sequencing; enrichment; DNA barcodes; Genetics; Genetik;

    Abstract : Advances in sequencing technologies constantly improves the throughput andaccuracy of sequencing instruments. Together with this development comes newdemands and opportunities to fully take advantage of the massive amounts of dataproduced within a sequence run. READ MORE

  5. 8. Distribution and activity of nitrogen-fixing bacteria in marine and estuarine waters

    Author : Hanna Farnelid; Lasse Riemann; Jonathan Zehr; Linnéuniversitetet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; 454-pyrosequencing; diazotrophs; heterotrophic bacteria; marine bacteria; marine microbial ecology; microbiology; nifH; nitrogenase; nitrogen fixation; PCR; qPCR; Mikrobiologi; Microbiology;

    Abstract : In aquatic environments the availability of nitrogen (N) generally limits primary production. N2-fixing prokaryotes (diazotrophs) can convert N2 gas into ammonium and provide significant input of N into the oceans. Cyanobacteria are thought to be the main N2-fixers but diazotrophs also include a wide range of heterotrophic bacteria. READ MORE

  6. 9. Droplet microfluidics for single cell and nucleic acid analysis

    Author : Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

    Abstract : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. READ MORE

  7. 10. Laboratory diagnosis of Bordetella infection : DNA hybridization and PCR validated against serology and culture in a phase 3 vaccine efficacy trial

    Author : Elisabet Reizenstein; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : The present study has focused on developing and validating assays alternative to culture for detection of Bordetella pertussis. The sensitivity of culture is comparatively low when compared to serology, varying from 30% to 80%. An attempt to increase this sensitivity was made by development of assays detecting the DNA of the bacteria. READ MORE