Search for dissertations about: "Enzyme assays"

Showing result 1 - 5 of 199 swedish dissertations containing the words Enzyme assays.

  2. 1. Enzyme assays for lignin–carbohydrate bond hydrolysis

    Author : Hampus Sunner; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Enzyme kinetic param- eter estimation; Glucuronoyl esterase; Enzyme assays; Lignin-carbohydrate bonds; Synergistic enzyme assay;

    Abstract : To identify, produce, and use enzymes, analytical methods known as enzyme assays are employed. Enzyme assays are based on analysing the changes brought about on a substrate by an enzyme under defined conditions. Assays are often based on simplified reactions acting as substitutes for the reaction of interest. READ MORE

  4. 2. Amine Transaminases in Biocatalytic Amine Synthesis

    Author : Henrik Land; Per Berglund; Helen C. Hailes; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Biocatalysis; Transamination; Reductive Amination; Enzyme; Enzyme Engineering; Equilibrium Displacement; Screening; Enzyme Stability; Bioteknologi; Biotechnology;

    Abstract : The use of enzymes, nature´s own catalysts, both isolated or as whole cells to perform chemical transformations is called biocatalysis. As a complement to classical chemical catalysis, biocatalysis can be an environmentally friendly and more economical option in the production and synthesis of chemicals. READ MORE

  5. 3. Combinatorial protein engineering applied to enzyme catalysis and molecular recognition

    Author : Malin Eklund; KTH; []
    Keywords : phage display; selection; enzyme; affinity domains; crystal structure; affibody; lipase; protein engineering; protein A; assembly;

    Abstract : The recent development of methods for constructing andhandling large collections (libraries) of proteins, from whichvariants with desired traits can be isolated, hasrevolutionized the field of protein engineering. Key elementsof such methods are the various ways in which the genotypes(the genes) and the phenotypes (the encoded proteins) arephysically linked during the process. READ MORE

  6. 4. Gene therapy tools: oligonucleotides and peptides

    Author : Jonas Eriksson; Ülo Langel; Jens Kurreck; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Gene therapy; oligonucleotide; peptide; RNA-cleaving deoxyribozyme; deoxyribozyme; DNAzyme; cell-penetrating peptide; CPP; enzyme; enzyme kinetics; kinetic assay; assay; telomerase; telomerase inhibitor; imetelstat; GRN163L; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Abstract : Genetic mutations can cause a wide range of diseases, e.g. cancer. Gene therapy has the potential to alleviate or even cure these diseases. READ MORE

  7. 5. Experimental and computational exploration of enzyme sequence space

    Author : Elzbieta Rembeza; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; high-throughput-screening; protein annotation; enzyme discovery; enzyme sequence space; oxidases;

    Abstract : Millions of enzymes with desirable features or new exciting activities can be found in organisms occupying diverse niches all around the earth. However, enzyme studies tend to be biased towards characterisation of representatives from eukaryotes, model organisms, or disease-causing bacteria. READ MORE