Search for dissertations about: "Enzyme isolation"

Showing result 1 - 5 of 44 swedish dissertations containing the words Enzyme isolation.

2. 1. Standardization of Islet Isolation and Transplantation Variables

   Author : Andrew S Friberg; Olle Korsgren; Daniel Brandhorst; Paul R. V. Johnson; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Islet isolation; standardization; enzyme; gradient separation; digital imaging analysis; DIA; transplantation outcome; islet transplantation; prediction; Medical technology; Medicinsk teknik; Medical Cell Biology; Medicinsk cellbiologi; Computerized Image Processing; Datoriserad bildbehandling;

   Abstract : Currently, the transplantation of islets of Langerhans is a viable means to maintain control of blood sugar levels and reduce the risk of hypoglycemia in defined populations with brittle type I diabetes mellitus or those requiring pancreatectomy. However, the process of islet isolation is highly variable and not all isolations result in islet numbers or quality suitable for transplantation. READ MORE

4. 2. Studies on Cytotoxic and Neutrophil Challenging Polypeptides and Cardiac Glycosides of Plant Origin

   Author : Senia Johansson; Tore Mellstrand; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical chemistry; cytotoxic; neutrophil; polypeptides; Phoradendron tomentosum; Digitalis purpurea; plant; mistletoe; MS MS; Edman degradation; breast cancer; macrocyclic peptides; multidrug resistance protein; primary structures; phoratoxin; isolation; digitoxin; cardiac lycoside; structure-activity; Farmaceutisk kemi; Pharmaceutical chemistry; Farmaceutisk kemi; Pharmacognosy; farmakognosi;

   Abstract : This thesis examines the isolation and characterisation (biological and chemical) of polypeptides from plants. A fractionation protocol was developed and applied on 100 plant materials with the aim of isolating highly purified polypeptide fractions from small amounts of plant materials. READ MORE

5. 3. Amine Transaminases in Multi-Step One-Pot Reactions

   Author : Mattias Anderson; Per Berglund; Frank Hollmann; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biocatalysis; enzyme; amine transaminase; ω-transaminase; amination; primary amine; chiral amine; chemoenzymatic; green chemistry; synthesis; cascade; Bioteknologi; Biotechnology;

   Abstract : Amine transaminases are enzymes that catalyze the mild and selective formation of primary amines, which are useful building blocks for biologically active compounds and natural products. In order to make the production of these kinds of compounds more efficient from both a practical and an environmental point of view, amine transaminases were incorporated into multi-step one-pot reactions. READ MORE

6. 4. Technical challenges in human islet isolation

   Author : Magnus Ståhle; Olle Korsgren; Andrew Friberg; Federico Bertuzzi; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

   Abstract : Transplantation of islets of Langerhans is an effective treatment option for patients with brittle type 1 diabetes mellitus. This treatment restores glucose control and also reduces hypoglycemia. Unfortunately, the outcome from islet isolations is variable, and many preparations do not yield sufficient islet number or islet quality. READ MORE

7. 5. Isolation and characterization of lipid-associated and neurosecretory polypeptides

   Author : Margareta Stark; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Lipid-interacting proteins play important roles in all living organisms. This thesis focuses on isolation and characterization of an enzyme in the triacylglycerol biosynthesis (phosphatidic acid phosphatase, PAP), hydrophobic polypeptides in bile, and polypeptides in cerebrospinal fluid. READ MORE