Search for dissertations about: "Enzyme synergy"

Showing result 1 - 5 of 15 swedish dissertations containing the words Enzyme synergy.

  1. 1. Enzyme assays for lignin–carbohydrate bond hydrolysis

    Author : Hampus Sunner; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Enzyme kinetic param- eter estimation; Glucuronoyl esterase; Enzyme assays; Lignin-carbohydrate bonds; Synergistic enzyme assay;

    Abstract : To identify, produce, and use enzymes, analytical methods known as enzyme assays are employed. Enzyme assays are based on analysing the changes brought about on a substrate by an enzyme under defined conditions. Assays are often based on simplified reactions acting as substitutes for the reaction of interest. READ MORE

  2. 2. Dietary fibre production from oat hulls

    Author : Eva Schmitz; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Oat hulls; Dietary fibre; Chemical composition; Bleaching; Pre-treatment; Enzyme synergy;

    Abstract : The current threats of climate change connected to the unsustainable consumption of fossil resources paired with an increasing world population demands the exploration of alternative raw materials for the production of food, fuel and specialty chemicals. Agricultural waste streams present suitable sources due to their availability in large quantities at low costs without competing with current food production. READ MORE

  3. 3. Exploring beta-mannan acting glycosidase families in hydrolysis and mannosyl transfer

    Author : Simon Birgersson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; glycoside hydrolases; beta-mannanase; Transglycosylation; enzymatic synthesis; Enzyme synergy; enzyme engineering; MALDI-TOF; HPLC; NMR;

    Abstract : The thesis revolves around the study of glycoside hydrolases (GH), specifically GH5 β-mannanases and GH36 α-galactosidase (I-III), as well as a GH130 mannoside phosphorylase (I, IV). These enzymes are investigated for their actions on and conversion of hemicellulosic β-mannan, including food-grade galactomannan and softwood galactoglucomannan, along with manno-oligosaccharides (MOS) derived from such polymers. READ MORE

  4. 4. Fungal cellulases: Study of hydrolytic properties of endoglucanases from Trichoderma reesei and Humicola insolens

    Author : Johan Karlsson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Humicola insolens; Trichoderma reesei; endoglucanases; cellulose hydrolysis; carboxymethyl cellulose; synergy; Biochemistry; Biokemi; Metabolism; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

    Abstract : Cellulose is one of the most abundant biopolymers on earth. Several organisms degrade cellulose, one of the most studied is the fungus Trichoderma reesei which produces seven genetically different cellulases. READ MORE

  5. 5. Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases

    Author : Mathias Wiemann; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; alpha-galactosidase; enzyme engineering; transglycosylation; MALDI-TOF MS; HPLC;

    Abstract : Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently attached to the enzyme, some GHs are able to catalyse synthesis reactions called transglycosylation. READ MORE