Search for dissertations about: "Enzymteknik"

Showing result 11 - 15 of 22 swedish dissertations containing the word Enzymteknik.

  1. 11. Rational engineering of esterases for improved amidase specificity in amide synthesis and hydrolysis

    Author : Peter Hendil-Forssell; Mats Martinelle; Romas J. Kazlauskas; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Amidase; Biocatalysis; Enzyme; Esterase; Enzyme engineering; Lipase; Substrate specificity; Biotechnology; Bioteknologi;

    Abstract : Biocatalysis is an ever evolving field that uses enzymes or microorganisms for chemical synthesis. By utilizing enzymes that generally have evolved for specific reactions under mild conditions and temperatures, biocatalysis can be a more environmentally friendly option compared to traditional chemistry. READ MORE

  2. 12. Metaproteogenomics-guided enzyme discovery : Targeted identification of novel proteases in microbial communities

    Author : Mikaela Johansson; Martin Karlsson; Bengt-Harald Jonsson; Florian-Alexander Herbst; Linköpings universitet; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY;

    Abstract : Industrial biotechnology is a large and growing industry as it is part of establishing a “greener” and more sustainable bioeconomy-based society. Using enzymes as biocatalysts is a viable alternative to chemicals and energy intense industrial processes and is en route to a more sustainable industry. READ MORE

  3. 13. On Catalytic Mechanisms for Rational Enzyme Design Strategies

    Author : Charlotte Kürten; Per-Olof Syrén; Rudolf Allemann; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; catalytic mechanisms; terpene cyclase; triterpene cyclase; solvent dynamics; protein hydration; thermodynamics; quantum tunneling; polycyclization; natural compounds; ? ?-hydrolase; esterase; amidase; enzyme engineering; biocatalysis; Biotechnology; Bioteknologi;

    Abstract : Enzymes enable life by promoting chemical reactions that govern the metabolism of all living organisms. As green catalysts, they have been extensively used in industry. However, to reach their full potential, engineering is often required, which can benefit from a detailed understanding of the underlying reaction mechanism. READ MORE

  4. 14. Transglycosylation by Glycoside Hydrolases - Production and modification of alkyl glycosides

    Author : Pontus Lundemo; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Glycoside hydrolases; Cyclodextrin glucanotransferases; β-glucosidases; Alkyl glycosides; Thermotoga neapolitana; Organic reaction media;

    Abstract : To enable the transition to a green, bio-based economy, an extensive enzymatic toolbox competitive to traditional chemical procedures is needed. One strong area for enzymes is carbohydrate chemistry, due to the over-functionalized nature of carbohydrates, difficult to handle in traditional chemistry. READ MORE

  5. 15. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity

    Author : Olof Modén; Bengt Mannervik; Mikael Widersten; Kenneth Tew; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURVETENSKAP; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; ENGINEERING AND TECHNOLOGY; allelic variants; azathioprine; bioactivation; chimeric mutagenesis; directed evolution; DNA shuffling; enzyme engineering; glutathione transferase; GST; lysate screening; molecular docking; multiple alignment; multivariate analysis; polymorphism; principal component analysis; prodrug; prodrug activation; protein engineering; protein redesign; reduced amino acid alphabet; saturation mutagenesis; semi-rational enzyme engineering; site-directed mutagenesis; structure-activity relationship; structure-based redesign; Biokemi; Biochemistry;

    Abstract : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. READ MORE