Search for dissertations about: "Ernst H Oliw"

Showing result 1 - 5 of 7 swedish dissertations containing the words Ernst H Oliw.

  2. 1. Expression of Manganese Lipoxygenase and Site-Directed Mutagenesis of Catalytically Important Amino Acids : Studies on Fatty Acid Dioxygenases

    Author : Mirela Cristea; Ernst H. Oliw; Chao Su; Olof Rådmark; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; Dioxygenase; Gaeumannomyces graminis; lipoxygenase; Magnaporthe grisea; oxylipin; Pichia pastoris; hydroperoxide isomerase; polyunsaturated fatty acids; Farmaceutisk farmakologi; Pharmaceutical pharmacology; Farmaceutisk farmakologi;

    Abstract : Polyunsaturated fatty acids can be bioactivated by two families of dioxygenases, which either contain non-heme iron (lipoxygenases) or heme (cyclooxygenases, linoleate diol synthases and α-dioxygenases).Lipoxygenases and their products play important roles in the pathophysiology of plants and fungi. READ MORE

  4. 2. Discovery of Novel Fatty Acid Dioxygenases and Cytochromes P450 : Mechanisms of Oxylipin Biosynthesis in Pathogenic Fungi

    Author : Inga Hoffmann; Ernst H Oliw; Alan R Brash; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Fusion protein; Linoleate diol synthase; Allene oxide synthase; Cyclooxygenase; Oxygenase; HPLC; Mass spectrometry; Hydroperoxide isomerase; Aspergillus; Fusarium oxysporum; Pharmaceutical Biochemistry; Farmaceutisk biokemi; Farmaceutisk farmakologi; Pharmaceutical Pharmacology; Farmaceutisk vetenskap; Pharmaceutical Science;

    Abstract : Dioxygenase-cytochrome P450 (DOX-CYP) fusion enzymes are present in diverse human and plant pathogenic fungi. They oxygenate fatty acids to lipid mediators which have regula­tory functions in fungal development and toxin production. READ MORE

  5. 3. Catalytic Properties and Tissue Distribution of Cytochrome P450 4F8 and 4F12 : Expression of CYP4F8 in Eye Tissues and Psoriatic Lesions

    Author : Katarina Stark; Ernst H Oliw; Leif Bertilsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; Arachidonic acid metabolism; Cytochrome P450; 19-Hydroxyprostaglandins; Immunohistochemistry; Ocular tissues; Psoriasis; Farmaceutisk farmakologi; Pharmaceutical pharmacology; Farmaceutisk farmakologi;

    Abstract : The human cytochrome P450 (CYP) family of monooxygenases is important for metabolism of drugs and endogenous compounds, e.g., vitamin A and D, cholesterol, steroids, fatty acids, and eicosanoids. This thesis describes the tissue distribution, catalytic properties, and possible function of CYP4F8 and CYP4F12. READ MORE

  6. 4. The structural basis for the catalytic specificity of manganese lipoxygenases : 3D structure analysis of the lipoxygenase of Magnaporthe oryzae

    Author : Anneli Wennman; Ernst H. Oliw; Marcia Newcomer; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; oxylipin; lipoxygenase; crystal structure; crystallography; HPLC; mass spectrometry; yeast; site-directed mutagenesis; fungi; Biochemical Pharmacology; Biokemisk farmakologi;

    Abstract : Lipoxygenases (LOX) catalyze regio- and stereospecific oxygenation of polyunsaturated fatty acids to hydroperoxides. These hydroperoxides are further metabolized to leukotrienes and lipoxins in mammals, and are involved in asthma and inflammation. LOX of animals and plants contain iron as catalytic metal (FeLOX). READ MORE

  7. 5. Catalytic and Structural Properties of Heme-containing Fatty Acid Dioxygenases : Similarities of Fungal Dioxygenases and Cyclooxygenases

    Author : Ulrike Garscha; Ernst Oliw; Per-Johan Jakobsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; dioxygenase; prostaglandin H synthase; Gaeumannomyces graminis; Aspergilli; hydroperoxide isomerase; linoleate diol synthase; cytochrome P450; oxylipin; Pharmaceutical pharmacology; Farmaceutisk farmakologi; Farmaceutisk farmakologi; Pharmaceutical Pharmacology;

    Abstract : 7,8-Linoleate diol synthase (7,8-LDS) of the take-all pathogen of wheat, Gaeumannomyces graminis, converts linoleic acid to 8R-hydroperoxyoctadecadienoic acid (8-HPODE) by 8-dioxygenase activity (8-DOX), and further isomerizes the hydroperoxide to 7S,8S-dihydroxyoctadecadienoic acid (7,8-DiHODE) by hydroperoxide isomerase activity. Sequence alignment showed homology to prostaglandin H synthase (PGHS), and both enzymes share structural and catalytic properties. READ MORE