Search for dissertations about: "Escherichia coli chemistry metabolism"

Showing result 1 - 5 of 7 swedish dissertations containing the words Escherichia coli chemistry metabolism.

  1. 1. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    University dissertation from Johan Nord, Department of Biochemistry, Center for Chemistry and Chemical Engineering, P.O. Box 124, S-221 00, Lund, Sweden

    Author : Johan Nord; Lunds universitet.; Lund University.; [2000]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Abstract : Popular Abstract in Swedish I plan to add a summary in Swedish at the WWW-page www.biokem.lu.se/education/theses/johannordthesis. READ MORE

  2. 2. Impact of glucose feed rate on productivity and recombinant protein quality in Escherichia coli

    University dissertation from Stockholm : KTH

    Author : Anna Maria Sandén; KTH.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Escherichia coli; recombinant protein production; fed-batch; feed profile; specific growth rate; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi;

    Abstract : The goal of this work was to contribute to the fed-batch process optimisation task by deriving parameters that have considerable impact on productivity as well as product quality The chosen parameters were I) the design of the glucose feed profile, II) the choice of induction strategy, with respect to the method of addition, and III) the time of the induction, with respect to the specific glucose consumption rate.The present fed-batch experiments using the lacUV5-promoter, for production of b-galactosidase, have shown that a high glucose feed rate gives a specific production rate, qp, that is twice as high, after induction, compared to a feed rate that is 2. READ MORE

  3. 3. Structural Studies of Microbial Proteins - From Escherichia coli and Herpesviruses

    University dissertation from Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Author : Daniel Gurmu; Stockholms universitet.; [2010]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CaiB; RibD; YhaK; SOX; ORF60; Herpesvirus; E. coli; MEDICINE Chemistry Biochemistry; MEDICIN Kemi Biokemi; biokemi; Biochemistry;

    Abstract : Structure biology concerns the study of the molecular structures of biological macromolecules, such as proteins, and how these relate to the function. Protein structures are also of importance in structure-based drug design. In this thesis, the work has been carried out in two different projects. READ MORE

  4. 4. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    University dissertation from Center for Chemistry and Chemical Engineering

    Author : Malin Mejàre; Lunds universitet.; Lund University.; [2000]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Abstract : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. READ MORE

  5. 5. Selective extraction systems for detection and purification of membrane proteins

    University dissertation from Ulf Sivars, Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University

    Author : Ulf Sivars; Lunds universitet.; Lund University.; [2000]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; partitioning; Aqueous two-phase systems; separation; membrane proteins; detergent; micelle; polymer; Biochemistry; affinity; Metabolism; Biokemi;

    Abstract : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. READ MORE